Hypoglycaemic Potentials of Frog oil in Alloxan-Induced Diabetic Rats

Osheke Shekins Okere; Moses Dele Adams; Uju Dorathy Ejike; Eunice Ogunwole; Ejike Daniel Eze

doi:doi:10.11648/j.ajbio.20140204.12

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Hypoglycaemic Potentials of Frog oil in Alloxan-Induced Diabetic Rats

Osheke Shekins Okere, Moses Dele Adams, Uju Dorathy Ejike, Eunice Ogunwole, Ejike Daniel Eze

Published in American Journal of BioScience (Volume 2, Issue 4)

Received: 4 June 2014 Accepted: 20 June 2014 Published: 30 June 2014

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Abstract

The hypoglycaemic potentials of frog oil in alloxan-induced diabetic rats were investigated. Thirty six male albino rats weighing 120.47±2.52 g was completely randomized into six groups (A-F) comprising 6 animals each. Animals in group A (control) were administered 1 ml of distilled water while those in groups B, C, D, E and F were induced with diabetes mellitus by intraperitoneal administration of alloxan monohydrate (180mg/kg body weight) and in addition were respectively administered distilled water, metformin (a reference antidiabetic drug), 3, 6 and 9 mg/kg body weight of frog oil once daily. Treatment with the oil lasted for 14 days during which blood glucose level and selected biochemical parameters were determined. The results showed that there was significant (p<0.05) reduction in glucose levels in the group treated with 9.0 mg/kg body weight of the oil from 221.22±0.15 to 100.15±0.07 mg/dl, indicating the best antidiabetic activity of all the treatment groups. The oil also caused significant (p<0.05) decrease in serum total bilirubin levels from 8.73±0.07 µmol/L to 2.43±0.03 µmol/L; serum total cholesterol levels from 313.48±0.05 mmol/L to 232.40±0.19 mmol/L; liver aspartate aminotransferase (AST) activity from 76.93±0.02 U/L to 35.25±0.02 U/L; liver alanine aminotransferase (ALT) activity from 85.52±0.05 U/L to 39.71±0.08 U/L respectively. Overall, these findings established the fact that frog oil has hypoglycaemic potentials and thus can be recommended for use in the treatment of diabetes. The results from biochemical parameters indicated that frog oil could also be explored in the control of some of the metabolic dysfunctions normally associated with diabetes.

Published in	American Journal of BioScience (Volume 2, Issue 4)
DOI	10.11648/j.ajbio.20140204.12
Page(s)	115-121
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Hypoglycaemic Potentials, Frog Oil, Alloxan, Diabetes Mellitus, Metformin, Blood Glucose

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APA Style

Osheke Shekins Okere, Moses Dele Adams, Uju Dorathy Ejike, Eunice Ogunwole, Ejike Daniel Eze. (2014). Hypoglycaemic Potentials of Frog oil in Alloxan-Induced Diabetic Rats. American Journal of BioScience, 2(4), 115-121. https://doi.org/10.11648/j.ajbio.20140204.12

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ACS Style

Osheke Shekins Okere; Moses Dele Adams; Uju Dorathy Ejike; Eunice Ogunwole; Ejike Daniel Eze. Hypoglycaemic Potentials of Frog oil in Alloxan-Induced Diabetic Rats. Am. J. BioScience 2014, 2(4), 115-121. doi: 10.11648/j.ajbio.20140204.12

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AMA Style

Osheke Shekins Okere, Moses Dele Adams, Uju Dorathy Ejike, Eunice Ogunwole, Ejike Daniel Eze. Hypoglycaemic Potentials of Frog oil in Alloxan-Induced Diabetic Rats. Am J BioScience. 2014;2(4):115-121. doi: 10.11648/j.ajbio.20140204.12

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@article{10.11648/j.ajbio.20140204.12,
  author = {Osheke Shekins Okere and Moses Dele Adams and Uju Dorathy Ejike and Eunice Ogunwole and Ejike Daniel Eze},
  title = {Hypoglycaemic Potentials of Frog oil in Alloxan-Induced Diabetic Rats},
  journal = {American Journal of BioScience},
  volume = {2},
  number = {4},
  pages = {115-121},
  doi = {10.11648/j.ajbio.20140204.12},
  url = {https://doi.org/10.11648/j.ajbio.20140204.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20140204.12},
  abstract = {The hypoglycaemic potentials of frog oil in alloxan-induced diabetic rats were investigated. Thirty six male albino rats weighing 120.47±2.52 g was completely randomized into six groups (A-F) comprising 6 animals each. Animals in group A (control) were administered 1 ml of distilled water while those in groups B, C, D, E and F were induced with diabetes mellitus by intraperitoneal administration of alloxan monohydrate (180mg/kg body weight) and in addition were respectively administered distilled water, metformin (a reference antidiabetic drug), 3, 6 and 9 mg/kg body weight of frog oil once daily. Treatment with the oil lasted for 14 days during which blood glucose level and selected biochemical parameters were determined. The results showed that there was significant (p<0.05) reduction in glucose levels in the group treated with 9.0 mg/kg body weight of the oil from 221.22±0.15 to 100.15±0.07 mg/dl, indicating the best antidiabetic activity of all the treatment groups. The oil also caused significant (p<0.05) decrease in serum total bilirubin levels from 8.73±0.07 µmol/L to 2.43±0.03 µmol/L; serum total cholesterol levels from 313.48±0.05 mmol/L to 232.40±0.19 mmol/L; liver aspartate aminotransferase (AST) activity from 76.93±0.02 U/L to 35.25±0.02 U/L; liver alanine aminotransferase (ALT) activity from 85.52±0.05 U/L to 39.71±0.08 U/L respectively. Overall, these findings established the fact that frog oil has hypoglycaemic potentials and thus can be recommended for use in the treatment of diabetes. The results from biochemical parameters indicated that frog oil could also be explored in the control of some of the metabolic dysfunctions normally associated with diabetes.},
 year = {2014}
}

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TY  - JOUR
T1  - Hypoglycaemic Potentials of Frog oil in Alloxan-Induced Diabetic Rats
AU  - Osheke Shekins Okere
AU  - Moses Dele Adams
AU  - Uju Dorathy Ejike
AU  - Eunice Ogunwole
AU  - Ejike Daniel Eze
Y1  - 2014/06/30
PY  - 2014
N1  - https://doi.org/10.11648/j.ajbio.20140204.12
DO  - 10.11648/j.ajbio.20140204.12
T2  - American Journal of BioScience
JF  - American Journal of BioScience
JO  - American Journal of BioScience
SP  - 115
EP  - 121
PB  - Science Publishing Group
SN  - 2330-0167
UR  - https://doi.org/10.11648/j.ajbio.20140204.12
AB  - The hypoglycaemic potentials of frog oil in alloxan-induced diabetic rats were investigated. Thirty six male albino rats weighing 120.47±2.52 g was completely randomized into six groups (A-F) comprising 6 animals each. Animals in group A (control) were administered 1 ml of distilled water while those in groups B, C, D, E and F were induced with diabetes mellitus by intraperitoneal administration of alloxan monohydrate (180mg/kg body weight) and in addition were respectively administered distilled water, metformin (a reference antidiabetic drug), 3, 6 and 9 mg/kg body weight of frog oil once daily. Treatment with the oil lasted for 14 days during which blood glucose level and selected biochemical parameters were determined. The results showed that there was significant (p<0.05) reduction in glucose levels in the group treated with 9.0 mg/kg body weight of the oil from 221.22±0.15 to 100.15±0.07 mg/dl, indicating the best antidiabetic activity of all the treatment groups. The oil also caused significant (p<0.05) decrease in serum total bilirubin levels from 8.73±0.07 µmol/L to 2.43±0.03 µmol/L; serum total cholesterol levels from 313.48±0.05 mmol/L to 232.40±0.19 mmol/L; liver aspartate aminotransferase (AST) activity from 76.93±0.02 U/L to 35.25±0.02 U/L; liver alanine aminotransferase (ALT) activity from 85.52±0.05 U/L to 39.71±0.08 U/L respectively. Overall, these findings established the fact that frog oil has hypoglycaemic potentials and thus can be recommended for use in the treatment of diabetes. The results from biochemical parameters indicated that frog oil could also be explored in the control of some of the metabolic dysfunctions normally associated with diabetes.
VL  - 2
IS  - 4
ER  -

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Author Information

Osheke Shekins Okere

Department of Biochemistry, Faculty of Science and Technology, Bingham University, Karu, Nasarawa State, Nigeria
Moses Dele Adams

Department of Biochemistry, Faculty of Science and Technology, Bingham University, Karu, Nasarawa State, Nigeria
Uju Dorathy Ejike

Department of Biochemistry, Faculty of Science and Technology, Bingham University, Karu, Nasarawa State, Nigeria
Eunice Ogunwole

Department of Physiology, Faculty of Basic Medical Sciences, Bingham University, Karu, Nasarawa State, Nigeria
Ejike Daniel Eze

Department of Physiology, Faculty of Basic Medical Sciences, Bingham University, Karu, Nasarawa State, Nigeria

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APA Style

Osheke Shekins Okere, Moses Dele Adams, Uju Dorathy Ejike, Eunice Ogunwole, Ejike Daniel Eze. (2014). Hypoglycaemic Potentials of Frog oil in Alloxan-Induced Diabetic Rats. American Journal of BioScience, 2(4), 115-121. https://doi.org/10.11648/j.ajbio.20140204.12

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ACS Style

Osheke Shekins Okere; Moses Dele Adams; Uju Dorathy Ejike; Eunice Ogunwole; Ejike Daniel Eze. Hypoglycaemic Potentials of Frog oil in Alloxan-Induced Diabetic Rats. Am. J. BioScience 2014, 2(4), 115-121. doi: 10.11648/j.ajbio.20140204.12

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AMA Style

Osheke Shekins Okere, Moses Dele Adams, Uju Dorathy Ejike, Eunice Ogunwole, Ejike Daniel Eze. Hypoglycaemic Potentials of Frog oil in Alloxan-Induced Diabetic Rats. Am J BioScience. 2014;2(4):115-121. doi: 10.11648/j.ajbio.20140204.12

Copy | Download

@article{10.11648/j.ajbio.20140204.12,
  author = {Osheke Shekins Okere and Moses Dele Adams and Uju Dorathy Ejike and Eunice Ogunwole and Ejike Daniel Eze},
  title = {Hypoglycaemic Potentials of Frog oil in Alloxan-Induced Diabetic Rats},
  journal = {American Journal of BioScience},
  volume = {2},
  number = {4},
  pages = {115-121},
  doi = {10.11648/j.ajbio.20140204.12},
  url = {https://doi.org/10.11648/j.ajbio.20140204.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20140204.12},
  abstract = {The hypoglycaemic potentials of frog oil in alloxan-induced diabetic rats were investigated. Thirty six male albino rats weighing 120.47±2.52 g was completely randomized into six groups (A-F) comprising 6 animals each. Animals in group A (control) were administered 1 ml of distilled water while those in groups B, C, D, E and F were induced with diabetes mellitus by intraperitoneal administration of alloxan monohydrate (180mg/kg body weight) and in addition were respectively administered distilled water, metformin (a reference antidiabetic drug), 3, 6 and 9 mg/kg body weight of frog oil once daily. Treatment with the oil lasted for 14 days during which blood glucose level and selected biochemical parameters were determined. The results showed that there was significant (p<0.05) reduction in glucose levels in the group treated with 9.0 mg/kg body weight of the oil from 221.22±0.15 to 100.15±0.07 mg/dl, indicating the best antidiabetic activity of all the treatment groups. The oil also caused significant (p<0.05) decrease in serum total bilirubin levels from 8.73±0.07 µmol/L to 2.43±0.03 µmol/L; serum total cholesterol levels from 313.48±0.05 mmol/L to 232.40±0.19 mmol/L; liver aspartate aminotransferase (AST) activity from 76.93±0.02 U/L to 35.25±0.02 U/L; liver alanine aminotransferase (ALT) activity from 85.52±0.05 U/L to 39.71±0.08 U/L respectively. Overall, these findings established the fact that frog oil has hypoglycaemic potentials and thus can be recommended for use in the treatment of diabetes. The results from biochemical parameters indicated that frog oil could also be explored in the control of some of the metabolic dysfunctions normally associated with diabetes.},
 year = {2014}
}

Copy | Download

TY  - JOUR
T1  - Hypoglycaemic Potentials of Frog oil in Alloxan-Induced Diabetic Rats
AU  - Osheke Shekins Okere
AU  - Moses Dele Adams
AU  - Uju Dorathy Ejike
AU  - Eunice Ogunwole
AU  - Ejike Daniel Eze
Y1  - 2014/06/30
PY  - 2014
N1  - https://doi.org/10.11648/j.ajbio.20140204.12
DO  - 10.11648/j.ajbio.20140204.12
T2  - American Journal of BioScience
JF  - American Journal of BioScience
JO  - American Journal of BioScience
SP  - 115
EP  - 121
PB  - Science Publishing Group
SN  - 2330-0167
UR  - https://doi.org/10.11648/j.ajbio.20140204.12
AB  - The hypoglycaemic potentials of frog oil in alloxan-induced diabetic rats were investigated. Thirty six male albino rats weighing 120.47±2.52 g was completely randomized into six groups (A-F) comprising 6 animals each. Animals in group A (control) were administered 1 ml of distilled water while those in groups B, C, D, E and F were induced with diabetes mellitus by intraperitoneal administration of alloxan monohydrate (180mg/kg body weight) and in addition were respectively administered distilled water, metformin (a reference antidiabetic drug), 3, 6 and 9 mg/kg body weight of frog oil once daily. Treatment with the oil lasted for 14 days during which blood glucose level and selected biochemical parameters were determined. The results showed that there was significant (p<0.05) reduction in glucose levels in the group treated with 9.0 mg/kg body weight of the oil from 221.22±0.15 to 100.15±0.07 mg/dl, indicating the best antidiabetic activity of all the treatment groups. The oil also caused significant (p<0.05) decrease in serum total bilirubin levels from 8.73±0.07 µmol/L to 2.43±0.03 µmol/L; serum total cholesterol levels from 313.48±0.05 mmol/L to 232.40±0.19 mmol/L; liver aspartate aminotransferase (AST) activity from 76.93±0.02 U/L to 35.25±0.02 U/L; liver alanine aminotransferase (ALT) activity from 85.52±0.05 U/L to 39.71±0.08 U/L respectively. Overall, these findings established the fact that frog oil has hypoglycaemic potentials and thus can be recommended for use in the treatment of diabetes. The results from biochemical parameters indicated that frog oil could also be explored in the control of some of the metabolic dysfunctions normally associated with diabetes.
VL  - 2
IS  - 4
ER  -

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