Sanitary Biosecurity Test against Hospital Acquired Fungal Infections: The Role of Hemolymph from the Cochineal Insect

Fernando Garía-Gil De Muñoz; Ignacio Del Río-Dueñas; Rodrigo Ramos-Zúñiga; Fidel Hernández-Hernández; H. Raúl. Pérez-Gómez; Ana Macías-Ornelas; Ramiro López-Elizalde

doi:doi:10.11648/j.ajcem.20140205.12

Sanitary Biosecurity Test against Hospital Acquired Fungal Infections: The Role of Hemolymph from the Cochineal Insect

Fernando Garía-Gil De Muñoz, Ignacio Del Río-Dueñas, Rodrigo Ramos-Zúñiga, Fidel Hernández-Hernández, H. Raúl. Pérez-Gómez, Ana Macías-Ornelas, Ramiro López-Elizalde

Published in American Journal of Clinical and Experimental Medicine (Volume 2, Issue 5)

Received: 3 September 2014 Accepted: 19 September 2014 Published: 30 September 2014

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Abstract

Background: Fungal infections are significant risk factors for nosocomial infections. They are associated with environmental spores and they are potential colonizers in hospital infrastructure, instruments or specific vectors. Usually they are identified by means of microbiology and culture media for definitive diagnosis. The objective is to evaluate the usefulness of the application of a colorimetric assay that originates from an endemic insect in Mexico (Dactilopius Coccus costa); It implies a specific qualitative biochemical reaction. It is also available to be used as a quick field test in health control. Design: Prospective, transversal, descriptive, randomized sampling with control reference test. Methods: A transversal randomized sampling from surfaces, materials, solutions and organic-sanitary waste from different known risk areas in a hospital institution with a large number of patients. Samples were processed using the qualitative test, examined by colorimetric evaluations and compared with positive controls (zymosan and aspergillus spores). Results: Samples showed no evidence of fungal colonization, unlike controls, which resulted positive. The resulting sensitivity was 100%. Conclusions: First qualitative pilot test to be used in the health care field, which proved to be useful for the monitoring and timely detection of fungi of biomedical interest. The method is practical. This essay validates the potential use of a quick qualitative test for preventive control of fungal infections in hospitals.

Published in	American Journal of Clinical and Experimental Medicine (Volume 2, Issue 5)
DOI	10.11648/j.ajcem.20140205.12
Page(s)	97-102
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Carminic Acid, Dactylopius, Hemolymph, Intrahospital Infection, Fungal Infection Diagnosis

References

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Fernando Garía-Gil De Muñoz, Ignacio Del Río-Dueñas, Rodrigo Ramos-Zúñiga, Fidel Hernández-Hernández, H. Raúl. Pérez-Gómez, et al. (2014). Sanitary Biosecurity Test against Hospital Acquired Fungal Infections: The Role of Hemolymph from the Cochineal Insect. American Journal of Clinical and Experimental Medicine, 2(5), 97-102. https://doi.org/10.11648/j.ajcem.20140205.12

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ACS Style

Fernando Garía-Gil De Muñoz; Ignacio Del Río-Dueñas; Rodrigo Ramos-Zúñiga; Fidel Hernández-Hernández; H. Raúl. Pérez-Gómez, et al. Sanitary Biosecurity Test against Hospital Acquired Fungal Infections: The Role of Hemolymph from the Cochineal Insect. Am. J. Clin. Exp. Med. 2014, 2(5), 97-102. doi: 10.11648/j.ajcem.20140205.12

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AMA Style

Fernando Garía-Gil De Muñoz, Ignacio Del Río-Dueñas, Rodrigo Ramos-Zúñiga, Fidel Hernández-Hernández, H. Raúl. Pérez-Gómez, et al. Sanitary Biosecurity Test against Hospital Acquired Fungal Infections: The Role of Hemolymph from the Cochineal Insect. Am J Clin Exp Med. 2014;2(5):97-102. doi: 10.11648/j.ajcem.20140205.12

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@article{10.11648/j.ajcem.20140205.12,
  author = {Fernando Garía-Gil De Muñoz and Ignacio Del Río-Dueñas and Rodrigo Ramos-Zúñiga and Fidel Hernández-Hernández and H. Raúl. Pérez-Gómez and Ana Macías-Ornelas and Ramiro López-Elizalde},
  title = {Sanitary Biosecurity Test against Hospital Acquired Fungal Infections: The Role of Hemolymph from the Cochineal Insect},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {2},
  number = {5},
  pages = {97-102},
  doi = {10.11648/j.ajcem.20140205.12},
  url = {https://doi.org/10.11648/j.ajcem.20140205.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20140205.12},
  abstract = {Background: Fungal infections are significant risk factors for nosocomial infections. They are associated with environmental spores and they are potential colonizers in hospital infrastructure, instruments or specific vectors. Usually they are identified by means of microbiology and culture media for definitive diagnosis. The objective is to evaluate the usefulness of the application of a colorimetric assay that originates from an endemic insect in Mexico (Dactilopius Coccus costa); It implies a specific qualitative biochemical reaction. It is also available to be used as a quick field test in health control. Design: Prospective, transversal, descriptive, randomized sampling with control reference test. Methods: A transversal randomized sampling from surfaces, materials, solutions and organic-sanitary waste from different known risk areas in a hospital institution with a large number of patients. Samples were processed using the qualitative test, examined by colorimetric evaluations and compared with positive controls (zymosan and aspergillus spores). Results: Samples showed no evidence of fungal colonization, unlike controls, which resulted positive. The resulting sensitivity was 100%. Conclusions: First qualitative pilot test to be used in the health care field, which proved to be useful for the monitoring and timely detection of fungi of biomedical interest. The method is practical. This essay validates the potential use of a quick qualitative test for preventive control of fungal infections in hospitals.},
 year = {2014}
}

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TY - JOUR
T1 - Sanitary Biosecurity Test against Hospital Acquired Fungal Infections: The Role of Hemolymph from the Cochineal Insect
AU - Fernando Garía-Gil De Muñoz
AU - Ignacio Del Río-Dueñas
AU - Rodrigo Ramos-Zúñiga
AU - Fidel Hernández-Hernández
AU - H. Raúl. Pérez-Gómez
AU - Ana Macías-Ornelas
AU - Ramiro López-Elizalde
Y1 - 2014/09/30
PY - 2014
N1 - https://doi.org/10.11648/j.ajcem.20140205.12
DO - 10.11648/j.ajcem.20140205.12
T2 - American Journal of Clinical and Experimental Medicine
JF - American Journal of Clinical and Experimental Medicine
JO - American Journal of Clinical and Experimental Medicine
SP - 97
EP - 102
PB - Science Publishing Group
SN - 2330-8133
UR - https://doi.org/10.11648/j.ajcem.20140205.12
AB - Background: Fungal infections are significant risk factors for nosocomial infections. They are associated with environmental spores and they are potential colonizers in hospital infrastructure, instruments or specific vectors. Usually they are identified by means of microbiology and culture media for definitive diagnosis. The objective is to evaluate the usefulness of the application of a colorimetric assay that originates from an endemic insect in Mexico (Dactilopius Coccus costa); It implies a specific qualitative biochemical reaction. It is also available to be used as a quick field test in health control. Design: Prospective, transversal, descriptive, randomized sampling with control reference test. Methods: A transversal randomized sampling from surfaces, materials, solutions and organic-sanitary waste from different known risk areas in a hospital institution with a large number of patients. Samples were processed using the qualitative test, examined by colorimetric evaluations and compared with positive controls (zymosan and aspergillus spores). Results: Samples showed no evidence of fungal colonization, unlike controls, which resulted positive. The resulting sensitivity was 100%. Conclusions: First qualitative pilot test to be used in the health care field, which proved to be useful for the monitoring and timely detection of fungi of biomedical interest. The method is practical. This essay validates the potential use of a quick qualitative test for preventive control of fungal infections in hospitals.
VL - 2
IS - 5
ER -

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Author Information

Fernando Garía-Gil De Muñoz
Ignacio Del Río-Dueñas
Rodrigo Ramos-Zúñiga
Fidel Hernández-Hernández

Laboratory of Molecular Entomology, Department of Molecular Pathogenesis of CINESTAV-IPN, IPN 2508, Mexico, D. F
H. Raúl. Pérez-Gómez

Infectious Pathology Institute, Hospital Civil De Guadalajara, Guadalajara, Mexico
Ana Macías-Ornelas

Neuroscience Department, CUCS, University of Guadalajara, Guadalajara, Mexico
Ramiro López-Elizalde

Hospital Civil J. I. Menchaca, Guadalajara, Mexico

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Fernando Garía-Gil De Muñoz, Ignacio Del Río-Dueñas, Rodrigo Ramos-Zúñiga, Fidel Hernández-Hernández, H. Raúl. Pérez-Gómez, et al. (2014). Sanitary Biosecurity Test against Hospital Acquired Fungal Infections: The Role of Hemolymph from the Cochineal Insect. American Journal of Clinical and Experimental Medicine, 2(5), 97-102. https://doi.org/10.11648/j.ajcem.20140205.12

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ACS Style

Fernando Garía-Gil De Muñoz; Ignacio Del Río-Dueñas; Rodrigo Ramos-Zúñiga; Fidel Hernández-Hernández; H. Raúl. Pérez-Gómez, et al. Sanitary Biosecurity Test against Hospital Acquired Fungal Infections: The Role of Hemolymph from the Cochineal Insect. Am. J. Clin. Exp. Med. 2014, 2(5), 97-102. doi: 10.11648/j.ajcem.20140205.12

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AMA Style

Fernando Garía-Gil De Muñoz, Ignacio Del Río-Dueñas, Rodrigo Ramos-Zúñiga, Fidel Hernández-Hernández, H. Raúl. Pérez-Gómez, et al. Sanitary Biosecurity Test against Hospital Acquired Fungal Infections: The Role of Hemolymph from the Cochineal Insect. Am J Clin Exp Med. 2014;2(5):97-102. doi: 10.11648/j.ajcem.20140205.12

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@article{10.11648/j.ajcem.20140205.12,
  author = {Fernando Garía-Gil De Muñoz and Ignacio Del Río-Dueñas and Rodrigo Ramos-Zúñiga and Fidel Hernández-Hernández and H. Raúl. Pérez-Gómez and Ana Macías-Ornelas and Ramiro López-Elizalde},
  title = {Sanitary Biosecurity Test against Hospital Acquired Fungal Infections: The Role of Hemolymph from the Cochineal Insect},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {2},
  number = {5},
  pages = {97-102},
  doi = {10.11648/j.ajcem.20140205.12},
  url = {https://doi.org/10.11648/j.ajcem.20140205.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20140205.12},
  abstract = {Background: Fungal infections are significant risk factors for nosocomial infections. They are associated with environmental spores and they are potential colonizers in hospital infrastructure, instruments or specific vectors. Usually they are identified by means of microbiology and culture media for definitive diagnosis. The objective is to evaluate the usefulness of the application of a colorimetric assay that originates from an endemic insect in Mexico (Dactilopius Coccus costa); It implies a specific qualitative biochemical reaction. It is also available to be used as a quick field test in health control. Design: Prospective, transversal, descriptive, randomized sampling with control reference test. Methods: A transversal randomized sampling from surfaces, materials, solutions and organic-sanitary waste from different known risk areas in a hospital institution with a large number of patients. Samples were processed using the qualitative test, examined by colorimetric evaluations and compared with positive controls (zymosan and aspergillus spores). Results: Samples showed no evidence of fungal colonization, unlike controls, which resulted positive. The resulting sensitivity was 100%. Conclusions: First qualitative pilot test to be used in the health care field, which proved to be useful for the monitoring and timely detection of fungi of biomedical interest. The method is practical. This essay validates the potential use of a quick qualitative test for preventive control of fungal infections in hospitals.},
 year = {2014}
}

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