Isolation, Characterization and Identification of Endophytic Bacteria in Maize (Zea Mays L.) Cultivated on Acrisols of the Southeast of Vietnam

Dang Thi Ngoc Thanh; Cao Ngoc Diep

doi:doi:10.11648/j.ajls.20140204.16

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Isolation, Characterization and Identification of Endophytic Bacteria in Maize (Zea Mays L.) Cultivated on Acrisols of the Southeast of Vietnam

Dang Thi Ngoc Thanh, Cao Ngoc Diep

Published in American Journal of Life Sciences (Volume 2, Issue 4)

Received: 11 August 2014 Accepted: 19 August 2014 Published: 30 August 2014

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Abstract

Endophytic bacterial diversity in Maize plant cultivated on Acrisols of the Eastern of South Vietnam was studied. Maize material was collected from five sites (provinces/cities) of this region. Endophytic bacteria were isolated in two kinds of medium (LGI, NFb) together with 16S rRNA gene fragments amplified from DNA using eubacterial universal primers (p515FPL and p13B). A total of 301 isolates were isolated and all of them have ability of nitrogen fixation and phosphate solubilization together with IAA biosynthesis but there were 30 isolates having the best characteristics and they were identified as maize endophytes and nifH gene owners. The sequences from selected endophytic bacteria (30 isolates) showed high degrees of similarity to those of the GenBank references strains (between 97% and 100%). Among the selected isolates were 6 isolates belong to Bacillus (20.00%) and 24 isolates belong to Proteobacteria (80.00%) including 2 alpha-proteobacteria (7%), 6 beta-proteobacteria (20%), and 16 gamma-proteobacteria (53%). Based on Pi value (nucleotide diversity), Proteobacteria group had the highest Theta values and Theta values (per sequence) from S of SNP for DNA polymorphism were calculated for each group and Proteobacteria group had the highest values in comparison of two groups. From these results showed that 3 isolates including DTN1b (Azotobacter vinelandii), VTN2b (Bacillus subtilis) and VTN7 (Enterobacter cloacae) proposed as potential microbial inoculants or biofertilizers for sustainable corn production in poor Acrisols in Vietnam because of their benefit and biosafety.

Published in	American Journal of Life Sciences (Volume 2, Issue 4)
DOI	10.11648/j.ajls.20140204.16
Page(s)	224-233
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Acrisols, 16S rRNA Gene Sequence, Endophytic Bacteria, Maize, the Eastern of South Vietnam

References

[1]	L. Arruda, A. Beneduzi, A. Martin, B. Lisboa, C. Lopes, F. Bertolo, L. M. P. Passaglis, and L.K. Vargas, “Screening of rhizobacteria isolated from maize (Zea mays L.) in Rio Grande do Sul State (South Brasil) and analysis of their potential to improve plant growth,” Applied Soil Ecology, vol. 63, pp. 15-22, 2013.
[2]	I.R. Kennedy, A.T.M.A. Choudhury, and M.L. Kecskes, “Non-symbiotic bacterial diazotrophs in crop-farming systems: can their potential for plant growth promotion be better exploited? Soil Biol. Biochem., vol. 36, pp.129-1244, 2004.
[3]	M.J. Jacobs, W.M. Bugbee, and D.A. Gabrielson, “Emuration, location, and characterization of endophytic bacteria within sugar beet roots,” Can.J. Botany., vol.63, pp.1262-1265, 1985.
[4]	D.G. Patriquin and J. Dobereiner, “Light microscopy observation of terrazolium-reducing bacteria in the endorhizosphere of maize and other grasses in Brasil,” Can. J. Microbiol., vol.24, pp.734-742, 1978.
[5]	C.R. Bell, G.A. Dickie, W.L.G. Harvey, and J.W.Y.F. Chan, “Endophytic bacteria in grapevine,” Can. J. Microbiol., vol. 41, pp.46-53, 1995.
[6]	J.I. Baldani, L. Caruso, V.L.D. Baldani, S.R. Goi, and J. Dobereiner, “Recent advances in BNF with non-legume plants,” Soil. Biol. Biochem., vol. 29, pp.911-922, 1997.
[7]	R.M. Boddey, .C. de Oliveira, S. Urquiaga, V.M. Reis, F.L. de Olivares, V.L.D. Baldani, and J. Dobereiner, “Biological nitrogen fixation associated with sugarcane and rice: Contributions and Prospects for improvement,” Plant Soil, vol.174, pp.195-209, 1995.
[8]	S.C. Verma, J.K. Ladha and A.K. Tripathi, “Evaluation of plant growth promoting and colonization ability of endophytic diazotrophs from deep water rice,” J. Biotechnol., vol. 91, pp.127-141, 2001.
[9]	S, Wakelin, R. Warren, P. Harvey and M. Ryder, “Phosphate solubilization by Penicillium spp. closely associated with wheat roots,” Bio. Fert. Soils, vol. 40, pp.36-43, 2004.
[10]	S. Lee, M. Flores-Encarnation, M. Contreras-Zentella, L. Garcia-Flores, J.E. Escamilla and C. Kennedy, “Indole-3-acetic acid biosynthesis is deficient in Gluconacetobacter diazotrophicus strains with mutations in cychrome C biogenesis genes,” J. Bacteriol., vol. 186, pp.5384-5391, 2004.
[11]	J.M. Costa and J.E. Loper, “Characterization of siderophore production by the biological-control agent Enterobacter clocae,” Mol. Plant Microbe. Interact., vol.7, pp.440-448, 1994.
[12]	T.D. Ha, T.D. Thao, N.T. Khiem, M.X. Trieu, R.V. Gerpacio, and P.L. Pingali, “Maize in Vietnam: Production Systems, Constraints, and Research Priorities”, Mexico, D.F.: CIMMYT, 2004.
[13]	L.H. Ba, “Vietnam Soil Resource,” Education Publishing House, Vietnam, 2009.
[14]	A.R. Anuar, Z.H. Shemsuddin, and O. Yaacob, “Contribution of legume-N by nodulated groundnut for growth of maize on an acid soil,” Soil. Biochem., vol.27, pp.595-601. doi:1016/0038-0717(95)98637-4.
[15]	D.T.N. Thanh, and C.N. Diep, “Isolation and identification of rhizospheric bacteria in Acrisols of maize (Zea mays L.) in the eastern of South Vietnam. American Journal of Life Sciences. vol. 2, No. 2, pp.82-89, 2014. doi: 10.11648/j.ajls.20140202.18 (online). ISSN 2328 – 5737.
[16]	V.A. Cavalcante and J. Dobereiner, “A new acid tolerant nitrogen fixing bacterium associated with sugarcane,” Plant Soil, vol. 108, pp.23-31, 1988.
[17]	G. Kirchhof, V.M. Reis, J.J. Baldani, B. Eckert, J. Dobereiner, and A. Hartmann, “Occurrence, physiological and molecular analysis of endophytic diazotrophic bacteria in gramineous energy plants,” Plant Soil, vol. 194, pp.45-55, 1997.
[18]	M. Park, C. Kim, J. Yang, H. Lee, W. Shin, S. Kim and T. Sa, “Isolation and characterization of diazotrophic growth promoting bacteria from Gram rhizosphere of agricultural crops of Korea,” Microbiological Research, vol 160, pp. 127-133, 2005.
[19]	C.S. Nautiyal, “An efficient microbiological growth medium for screening phosphate-solubilizing microorganisms,” FEMS Microbiology Letters, vol. 170, pp.256-270, 1999.
[20]	S.A. Gordon and R.P. Weber., “Colometric estimation of indolacetic acid,” Plant Physiol., vol 26, pp.192-195, 1951.
[21]	B. Neumann, A. Pospiech, and H.U. Schairrer, “Rapid isolation of genomic DNA from Gram-negative,” Trends Gent., vol. 8, pp. 332-333, 1992.
[22]	D.A. Relman, T.M. Schmidt, E.P. MacDermontt, and S. Falkow, “Identification of the uncultured bacillus of Whipple’s disease,” N. Engl. J. Med., vol. 327, pp.293-301, 1992.
[23]	D.A. Relman, J.S. Loutit, T.M. Schmidt, S. Falkow, and L.S. Tompkin, “The agent of bacillary angiomatosis. An approach to the identification of uncultuted pathogens,” N. Engl. J. Med., vol. 323, pp.1573-1580, 1990.
[24]	K.D. Zinniel, P. Lambercht, N.B. Harris, Z. Feng, D. Kuczmarshki, P. Higley, C.A. Ishimaru, A. Arunakumari, R.G. Barletta, and A.K. Vidaver, “Isolation and charcaterization of endophytic bacteria from agronomic crops and prairie plants,” Appl. Environ. Microbiol., vol. 68, pp. 2198-2208, 2002.
[25]	K. Tamura, D. Peterson, N. Peterson, G. Stecher, M. Nei, and S. Kumar, “MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likehood, Evolutionary Distance and Maximum Parsimony Methods,” Mol. Biol. Evol., vol. 28, pp. 2731-2739, 2011.
[26]	F. Poly, L.M. Joteur, R. Bally, “Improvement in RELP procedure to study the community of nitrogen fixers in soil through the diversity of nifH gene.” Res. Microbiol., vol. 152, pp. 95-103, 2001.
[27]	M.K. Halushka, J.B. Fan, K. Bentley, L. Hsie, N. Shen, A. Weder, R. Cooper, R. Lipshutz, and A. Charavarti, “Patterns of single-nucleotide polymorphisms in candidate genes for blood-pressure homestasis,” Nat. Genet., vol. 22(3), pp. 239-247, 1999.
[28]	O.B. Weber, V.L.D. Baldani, K.R.S. Teixeira, G. Kirchof, J.I. Baldani, and J. Dobereiner, “Isolation and characterization of diazotrophic bacteria from banana and pineapple plants,” Plant and Soil., vol 210, pp. 103-113, 1999.
[29]	N.T.Thu Ha, H.T. Toan, C.N.Điep, “Isolation anf characrterization of endophytic bacteria in several forage grass cultivars,” J. Biotechnology., vol. 7(2), pp.241-250, 2009.
[30]	R.P. Ryan, K. Germanie, A. Franks, D. J. Ryan and D.N. Dowling, “Bacterial endophytes: recent developments and applications,” FEMS Microbiol. Lett., vol. 278, pp.1-9, 2008.
[31]	D.Y. Kobayashi, and J.D. Palumbo, “Bacterial Endophytes and Their Effects on Plants and Uses in Agricultulture,” C.W. Bacon and J.F. White. Eds, Marcel Dekker, New York, 2000.
[32]	C. Lodewyckx, J. Vangronsveld, F. Porteous, E.R.B. Moore, S. Taghavi, M. Mezgeay and D. van der Lelie, “Endophytic Bacteria and Their Potential Applications,” Critical Reviews in Plant Sciences, vol. 21 (6), pp. 583-602, 2002.
[33]	A. Montanez, A.R. Blanco, C.Barlocco, M. Beracochea, and M. Sicardi, “Characterization of cultivable putative endophytic plant growth promoting bacteria associated with maize cultivars (Zea mays L.) and their inoculation effects in vitro,” Applied Soil Ecology, vol.58, pp.21-28, 2012.
[34]	G.A. Watterson, “On the number of segregation sites in general models without recobination,” Theor. Pop. Biol., vol. 7, pp. 256-276, 1975.
[35]	J. Rozas, and R. Rozas, “DnaSP version 4.1: an integrated program for molecular population genetics and molecular evolution analysis,” Bioinformatics, vol. 15, pp. 174-175, 2005.
[36]	V. J. Szilagyi-Zecchin, A. C. Ikeda, M. Hungria, D. Adamoski, V. Kava-Cordeiro, C. Glienke, and L. V. Galli-Terasawa, “Identification and characterization of endophytic bacteria from corn (Zea mays L.) roots with biotechnological potential in agriculture”, AMB Express, vol.4(26). Published online: 7 May 2014. doi: 10.1186/s13568-014-0026-y.
[37]	N. Benhamou, S. Gagne, D. Le Quere, and E.M. Top, “Bacterial mediated induced resistance in cucumber: beneficial effect of the endophytic bacterium Serratia phymuhica on the protection against infection by Pythium ultimum,” Phytopathology, vol. 90, pp.45-56, 2000.
[38]	A.V. Sturz, and J. Nowak, “Endophytic communities of rhizobacteria and the strategies required to create yield enhancing associations with crops,” Appl. Soil. Ecol., vol.15, pp.183-190, 2000.
[39]	A.C. Ikeda, L.L. Bassani, D. Adamoski, D. Stringari, V.K. Cordeiro, C. Glienke, M.B. R. Steffens, M. Hungaria, L.V. Galli-Terasawa, “Morphological and genetic characterization of endophytic bacteria isolated from roots of different maize genotypes,” Microb. Ecol., vol. 65, pp.154-160, 2013.
[40]	J.A. McInroy, and J.W. Klorpper, “Survey of indigenous bacterial endophytic from cotton and sweet corn,” Plant Soil, vol. 173, pp.337-342, 1995.
[41]	D. Seghers, L. Wittebolle, E.M. Top, W. Verstraete, and S.D. Siliano, “Impact of Agricultural Practices on the Zea mays L. Endophytic Community,” Appl. Environ. Microbiol., vol.70(3), pp. 1475-1482, 2004.
[42]	R. Rai, P.K. Dash, B.M. Prasanna, and A. Singh,”Endophytic bacterial flora in the stem tissue of a tropical maize (Zea mays L.) genotype: isolation, identification and enumeration,” World J. Microbiol. Biotechnol.., vol. 23, pp. 853-858, 2007.
[43]	L.F.W. Roesch, F.A.O. Camargo, F.M. Bento, and E.W. Triplett, “Biodiversity of diazotrophic bacteria within the soil, rott and stem of field-grown maize,” Plant Soil, vol.302, pp.91-104, 2008.

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Dang Thi Ngoc Thanh, Cao Ngoc Diep. (2014). Isolation, Characterization and Identification of Endophytic Bacteria in Maize (Zea Mays L.) Cultivated on Acrisols of the Southeast of Vietnam. American Journal of Life Sciences, 2(4), 224-233. https://doi.org/10.11648/j.ajls.20140204.16

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ACS Style

Dang Thi Ngoc Thanh; Cao Ngoc Diep. Isolation, Characterization and Identification of Endophytic Bacteria in Maize (Zea Mays L.) Cultivated on Acrisols of the Southeast of Vietnam. Am. J. Life Sci. 2014, 2(4), 224-233. doi: 10.11648/j.ajls.20140204.16

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Dang Thi Ngoc Thanh, Cao Ngoc Diep. Isolation, Characterization and Identification of Endophytic Bacteria in Maize (Zea Mays L.) Cultivated on Acrisols of the Southeast of Vietnam. Am J Life Sci. 2014;2(4):224-233. doi: 10.11648/j.ajls.20140204.16

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@article{10.11648/j.ajls.20140204.16,
  author = {Dang Thi Ngoc Thanh and Cao Ngoc Diep},
  title = {Isolation, Characterization and Identification of Endophytic Bacteria in Maize (Zea Mays L.) Cultivated on Acrisols of the Southeast of Vietnam},
  journal = {American Journal of Life Sciences},
  volume = {2},
  number = {4},
  pages = {224-233},
  doi = {10.11648/j.ajls.20140204.16},
  url = {https://doi.org/10.11648/j.ajls.20140204.16},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20140204.16},
  abstract = {Endophytic bacterial diversity in Maize plant cultivated on Acrisols of the Eastern of South Vietnam was studied. Maize material was collected from five sites (provinces/cities) of this region. Endophytic bacteria were isolated in two kinds of medium (LGI, NFb) together with 16S rRNA gene fragments amplified from DNA using eubacterial universal primers (p515FPL and p13B). A total of 301 isolates were isolated and all of them have ability of nitrogen fixation and phosphate solubilization together with IAA biosynthesis but there were 30 isolates having the best characteristics and they were identified as maize endophytes and nifH gene owners. The sequences from selected endophytic bacteria (30 isolates) showed high degrees of similarity to those of the GenBank references strains (between 97% and 100%). Among the selected isolates were 6 isolates belong to Bacillus (20.00%) and 24 isolates belong to Proteobacteria (80.00%) including 2 alpha-proteobacteria (7%), 6 beta-proteobacteria (20%), and 16 gamma-proteobacteria (53%). Based on Pi value (nucleotide diversity), Proteobacteria group had the highest Theta values and Theta values (per sequence) from S of SNP for DNA polymorphism were calculated for each group and Proteobacteria group had the highest values in comparison of two groups. From these results showed that 3 isolates including DTN1b (Azotobacter vinelandii), VTN2b (Bacillus subtilis) and VTN7 (Enterobacter cloacae) proposed as potential microbial inoculants or biofertilizers for sustainable corn production in poor Acrisols in Vietnam because of their benefit and biosafety.},
 year = {2014}
}

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TY  - JOUR
T1  - Isolation, Characterization and Identification of Endophytic Bacteria in Maize (Zea Mays L.) Cultivated on Acrisols of the Southeast of Vietnam
AU  - Dang Thi Ngoc Thanh
AU  - Cao Ngoc Diep
Y1  - 2014/08/30
PY  - 2014
N1  - https://doi.org/10.11648/j.ajls.20140204.16
DO  - 10.11648/j.ajls.20140204.16
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 224
EP  - 233
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.20140204.16
AB  - Endophytic bacterial diversity in Maize plant cultivated on Acrisols of the Eastern of South Vietnam was studied. Maize material was collected from five sites (provinces/cities) of this region. Endophytic bacteria were isolated in two kinds of medium (LGI, NFb) together with 16S rRNA gene fragments amplified from DNA using eubacterial universal primers (p515FPL and p13B). A total of 301 isolates were isolated and all of them have ability of nitrogen fixation and phosphate solubilization together with IAA biosynthesis but there were 30 isolates having the best characteristics and they were identified as maize endophytes and nifH gene owners. The sequences from selected endophytic bacteria (30 isolates) showed high degrees of similarity to those of the GenBank references strains (between 97% and 100%). Among the selected isolates were 6 isolates belong to Bacillus (20.00%) and 24 isolates belong to Proteobacteria (80.00%) including 2 alpha-proteobacteria (7%), 6 beta-proteobacteria (20%), and 16 gamma-proteobacteria (53%). Based on Pi value (nucleotide diversity), Proteobacteria group had the highest Theta values and Theta values (per sequence) from S of SNP for DNA polymorphism were calculated for each group and Proteobacteria group had the highest values in comparison of two groups. From these results showed that 3 isolates including DTN1b (Azotobacter vinelandii), VTN2b (Bacillus subtilis) and VTN7 (Enterobacter cloacae) proposed as potential microbial inoculants or biofertilizers for sustainable corn production in poor Acrisols in Vietnam because of their benefit and biosafety.
VL  - 2
IS  - 4
ER  -

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Author Information

Dang Thi Ngoc Thanh

Dept. Natural Science Teacher Training, Sai Gon University, HCM city, Vietnam
Cao Ngoc Diep

Dept. Microbiology Biotechnology, Biotechnology R&D Institute, Can Tho University, Can Tho City, Vietnam

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Dang Thi Ngoc Thanh, Cao Ngoc Diep. (2014). Isolation, Characterization and Identification of Endophytic Bacteria in Maize (Zea Mays L.) Cultivated on Acrisols of the Southeast of Vietnam. American Journal of Life Sciences, 2(4), 224-233. https://doi.org/10.11648/j.ajls.20140204.16

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ACS Style

Dang Thi Ngoc Thanh; Cao Ngoc Diep. Isolation, Characterization and Identification of Endophytic Bacteria in Maize (Zea Mays L.) Cultivated on Acrisols of the Southeast of Vietnam. Am. J. Life Sci. 2014, 2(4), 224-233. doi: 10.11648/j.ajls.20140204.16

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AMA Style

Dang Thi Ngoc Thanh, Cao Ngoc Diep. Isolation, Characterization and Identification of Endophytic Bacteria in Maize (Zea Mays L.) Cultivated on Acrisols of the Southeast of Vietnam. Am J Life Sci. 2014;2(4):224-233. doi: 10.11648/j.ajls.20140204.16

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@article{10.11648/j.ajls.20140204.16,
  author = {Dang Thi Ngoc Thanh and Cao Ngoc Diep},
  title = {Isolation, Characterization and Identification of Endophytic Bacteria in Maize (Zea Mays L.) Cultivated on Acrisols of the Southeast of Vietnam},
  journal = {American Journal of Life Sciences},
  volume = {2},
  number = {4},
  pages = {224-233},
  doi = {10.11648/j.ajls.20140204.16},
  url = {https://doi.org/10.11648/j.ajls.20140204.16},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20140204.16},
  abstract = {Endophytic bacterial diversity in Maize plant cultivated on Acrisols of the Eastern of South Vietnam was studied. Maize material was collected from five sites (provinces/cities) of this region. Endophytic bacteria were isolated in two kinds of medium (LGI, NFb) together with 16S rRNA gene fragments amplified from DNA using eubacterial universal primers (p515FPL and p13B). A total of 301 isolates were isolated and all of them have ability of nitrogen fixation and phosphate solubilization together with IAA biosynthesis but there were 30 isolates having the best characteristics and they were identified as maize endophytes and nifH gene owners. The sequences from selected endophytic bacteria (30 isolates) showed high degrees of similarity to those of the GenBank references strains (between 97% and 100%). Among the selected isolates were 6 isolates belong to Bacillus (20.00%) and 24 isolates belong to Proteobacteria (80.00%) including 2 alpha-proteobacteria (7%), 6 beta-proteobacteria (20%), and 16 gamma-proteobacteria (53%). Based on Pi value (nucleotide diversity), Proteobacteria group had the highest Theta values and Theta values (per sequence) from S of SNP for DNA polymorphism were calculated for each group and Proteobacteria group had the highest values in comparison of two groups. From these results showed that 3 isolates including DTN1b (Azotobacter vinelandii), VTN2b (Bacillus subtilis) and VTN7 (Enterobacter cloacae) proposed as potential microbial inoculants or biofertilizers for sustainable corn production in poor Acrisols in Vietnam because of their benefit and biosafety.},
 year = {2014}
}

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TY  - JOUR
T1  - Isolation, Characterization and Identification of Endophytic Bacteria in Maize (Zea Mays L.) Cultivated on Acrisols of the Southeast of Vietnam
AU  - Dang Thi Ngoc Thanh
AU  - Cao Ngoc Diep
Y1  - 2014/08/30
PY  - 2014
N1  - https://doi.org/10.11648/j.ajls.20140204.16
DO  - 10.11648/j.ajls.20140204.16
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 224
EP  - 233
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.20140204.16
AB  - Endophytic bacterial diversity in Maize plant cultivated on Acrisols of the Eastern of South Vietnam was studied. Maize material was collected from five sites (provinces/cities) of this region. Endophytic bacteria were isolated in two kinds of medium (LGI, NFb) together with 16S rRNA gene fragments amplified from DNA using eubacterial universal primers (p515FPL and p13B). A total of 301 isolates were isolated and all of them have ability of nitrogen fixation and phosphate solubilization together with IAA biosynthesis but there were 30 isolates having the best characteristics and they were identified as maize endophytes and nifH gene owners. The sequences from selected endophytic bacteria (30 isolates) showed high degrees of similarity to those of the GenBank references strains (between 97% and 100%). Among the selected isolates were 6 isolates belong to Bacillus (20.00%) and 24 isolates belong to Proteobacteria (80.00%) including 2 alpha-proteobacteria (7%), 6 beta-proteobacteria (20%), and 16 gamma-proteobacteria (53%). Based on Pi value (nucleotide diversity), Proteobacteria group had the highest Theta values and Theta values (per sequence) from S of SNP for DNA polymorphism were calculated for each group and Proteobacteria group had the highest values in comparison of two groups. From these results showed that 3 isolates including DTN1b (Azotobacter vinelandii), VTN2b (Bacillus subtilis) and VTN7 (Enterobacter cloacae) proposed as potential microbial inoculants or biofertilizers for sustainable corn production in poor Acrisols in Vietnam because of their benefit and biosafety.
VL  - 2
IS  - 4
ER  -

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