Isolation, Characterization and Identification of Phosphate- and Potassium- Solubilizing Bacteria from Weathered Materials of Granite Rock Mountain, That Son, an Giang Province, Vietnam

Nguyen Thi Don; Cao Ngoc Diep

doi:doi:10.11648/j.ajls.20140205.16

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Isolation, Characterization and Identification of Phosphate- and Potassium- Solubilizing Bacteria from Weathered Materials of Granite Rock Mountain, That Son, an Giang Province, Vietnam

Nguyen Thi Don, Cao Ngoc Diep

Published in American Journal of Life Sciences (Volume 2, Issue 5)

Received: 9 October 2014 Accepted: 18 October 2014 Published: 30 October 2014

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Abstract

Two hundred and thirty-seven bacterial isolates from 118 sample soils/weathered granite rock of That Son Mountain, An Giang province, Vietnam were isolated on Aleksandrov medium. Their colonies were round or irregular, white to yellow and their shape was rod, motile. Thirty four of two hundred and thirty-seven bacterial isolates were capable of dissolving both phosphate and potassium and seven strains had high phosphate and potassium dissolution capacity (>15 mg l-1 P2O5 and >10 mg l-1 K2O). These thirty four strains were identified by using PCR technique with specific primers fd1 and rP and DNA sequencing. The results of DNAsequencing were compared with GenBank database of NCBI by BLAST N software. The sequences from selected phosphate and potassium isolates (34 isolates) showed high degrees of similarity to those of the GenBank references (between 99% and 100%). Among these sequenced isolates, 8 isolates belong to Bacilli (23.53%) and 26 isolates belong to Proteobacteria (76.47%) including 21 alpha-proteobacteria (61.76%) and 5 gamma-proteobacteria (14.71%). Based on Pi value (nucleotide diversity), Proteobacteria group had the highest Theta values and Theta values (per sequence) from S of SNP for DNA polymorphism were calculated for each group and AlphaProteobacteria group had the highest values in comparistion with two groups. These results showed that 3 strains including CA09 (Agrobacterium tumefaciens), CA29 (Rhizobium tropici) and K16B (Azotobacter tropicalis) proposed as potential microbial inoculants or biofertilizers for sustainable crop production in sandy acid soil in Vietnam because of their benefits and biosafety.

Published in	American Journal of Life Sciences (Volume 2, Issue 5)
DOI	10.11648/j.ajls.20140205.16
Page(s)	282-291
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Acinetobacter, Agrobacterium, Azotobacter, Bacillus, Identification, Phosphate and Potassium Solubilization, Rhizobium, Weathered Materials of Granite Rock

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Nguyen Thi Don, Cao Ngoc Diep. (2014). Isolation, Characterization and Identification of Phosphate- and Potassium- Solubilizing Bacteria from Weathered Materials of Granite Rock Mountain, That Son, an Giang Province, Vietnam. American Journal of Life Sciences, 2(5), 282-291. https://doi.org/10.11648/j.ajls.20140205.16

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Nguyen Thi Don; Cao Ngoc Diep. Isolation, Characterization and Identification of Phosphate- and Potassium- Solubilizing Bacteria from Weathered Materials of Granite Rock Mountain, That Son, an Giang Province, Vietnam. Am. J. Life Sci. 2014, 2(5), 282-291. doi: 10.11648/j.ajls.20140205.16

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AMA Style

Nguyen Thi Don, Cao Ngoc Diep. Isolation, Characterization and Identification of Phosphate- and Potassium- Solubilizing Bacteria from Weathered Materials of Granite Rock Mountain, That Son, an Giang Province, Vietnam. Am J Life Sci. 2014;2(5):282-291. doi: 10.11648/j.ajls.20140205.16

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@article{10.11648/j.ajls.20140205.16,
  author = {Nguyen Thi Don and Cao Ngoc Diep},
  title = {Isolation, Characterization and Identification of Phosphate- and Potassium- Solubilizing Bacteria from Weathered Materials of Granite Rock Mountain, That Son, an Giang Province, Vietnam},
  journal = {American Journal of Life Sciences},
  volume = {2},
  number = {5},
  pages = {282-291},
  doi = {10.11648/j.ajls.20140205.16},
  url = {https://doi.org/10.11648/j.ajls.20140205.16},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20140205.16},
  abstract = {Two hundred and thirty-seven bacterial isolates from 118 sample soils/weathered granite rock of That Son Mountain, An Giang province, Vietnam were isolated on Aleksandrov medium. Their colonies were round or irregular, white to yellow and their shape was rod, motile. Thirty four of two hundred and thirty-seven bacterial isolates were capable of dissolving both phosphate and potassium and seven strains had high phosphate and potassium dissolution capacity (>15 mg l-1 P2O5 and >10 mg l-1 K2O). These thirty four strains were identified by using PCR technique with specific primers fd1 and rP and DNA sequencing. The results of DNAsequencing were compared with GenBank database of NCBI by BLAST N software. The sequences from selected phosphate and potassium isolates (34 isolates) showed high degrees of similarity to those of the GenBank references (between 99% and 100%). Among these sequenced isolates, 8 isolates belong to Bacilli (23.53%) and 26 isolates belong to Proteobacteria (76.47%) including 21 alpha-proteobacteria (61.76%) and 5 gamma-proteobacteria (14.71%). Based on Pi value (nucleotide diversity), Proteobacteria group had the highest Theta values and Theta values (per sequence) from S of SNP for DNA polymorphism were calculated for each group and AlphaProteobacteria group had the highest values in comparistion with two groups. These results showed that 3 strains including CA09 (Agrobacterium tumefaciens), CA29 (Rhizobium tropici) and K16B (Azotobacter tropicalis) proposed as potential microbial inoculants or biofertilizers for sustainable crop production in sandy acid soil in Vietnam because of their benefits and biosafety.},
 year = {2014}
}

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TY  - JOUR
T1  - Isolation, Characterization and Identification of Phosphate- and Potassium- Solubilizing Bacteria from Weathered Materials of Granite Rock Mountain, That Son, an Giang Province, Vietnam
AU  - Nguyen Thi Don
AU  - Cao Ngoc Diep
Y1  - 2014/10/30
PY  - 2014
N1  - https://doi.org/10.11648/j.ajls.20140205.16
DO  - 10.11648/j.ajls.20140205.16
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 282
EP  - 291
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.20140205.16
AB  - Two hundred and thirty-seven bacterial isolates from 118 sample soils/weathered granite rock of That Son Mountain, An Giang province, Vietnam were isolated on Aleksandrov medium. Their colonies were round or irregular, white to yellow and their shape was rod, motile. Thirty four of two hundred and thirty-seven bacterial isolates were capable of dissolving both phosphate and potassium and seven strains had high phosphate and potassium dissolution capacity (>15 mg l-1 P2O5 and >10 mg l-1 K2O). These thirty four strains were identified by using PCR technique with specific primers fd1 and rP and DNA sequencing. The results of DNAsequencing were compared with GenBank database of NCBI by BLAST N software. The sequences from selected phosphate and potassium isolates (34 isolates) showed high degrees of similarity to those of the GenBank references (between 99% and 100%). Among these sequenced isolates, 8 isolates belong to Bacilli (23.53%) and 26 isolates belong to Proteobacteria (76.47%) including 21 alpha-proteobacteria (61.76%) and 5 gamma-proteobacteria (14.71%). Based on Pi value (nucleotide diversity), Proteobacteria group had the highest Theta values and Theta values (per sequence) from S of SNP for DNA polymorphism were calculated for each group and AlphaProteobacteria group had the highest values in comparistion with two groups. These results showed that 3 strains including CA09 (Agrobacterium tumefaciens), CA29 (Rhizobium tropici) and K16B (Azotobacter tropicalis) proposed as potential microbial inoculants or biofertilizers for sustainable crop production in sandy acid soil in Vietnam because of their benefits and biosafety.
VL  - 2
IS  - 5
ER  -

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Author Information

Nguyen Thi Don

Dept. Biology, College of Natural Science, Can Tho University, Can Tho City, Vietnam
Cao Ngoc Diep

Dept. Microbiology Biotechnology, Biotechnology R&D Institute, Can Tho University, Can Tho City, Vietnam

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Nguyen Thi Don, Cao Ngoc Diep. (2014). Isolation, Characterization and Identification of Phosphate- and Potassium- Solubilizing Bacteria from Weathered Materials of Granite Rock Mountain, That Son, an Giang Province, Vietnam. American Journal of Life Sciences, 2(5), 282-291. https://doi.org/10.11648/j.ajls.20140205.16

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ACS Style

Nguyen Thi Don; Cao Ngoc Diep. Isolation, Characterization and Identification of Phosphate- and Potassium- Solubilizing Bacteria from Weathered Materials of Granite Rock Mountain, That Son, an Giang Province, Vietnam. Am. J. Life Sci. 2014, 2(5), 282-291. doi: 10.11648/j.ajls.20140205.16

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AMA Style

Nguyen Thi Don, Cao Ngoc Diep. Isolation, Characterization and Identification of Phosphate- and Potassium- Solubilizing Bacteria from Weathered Materials of Granite Rock Mountain, That Son, an Giang Province, Vietnam. Am J Life Sci. 2014;2(5):282-291. doi: 10.11648/j.ajls.20140205.16

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@article{10.11648/j.ajls.20140205.16,
  author = {Nguyen Thi Don and Cao Ngoc Diep},
  title = {Isolation, Characterization and Identification of Phosphate- and Potassium- Solubilizing Bacteria from Weathered Materials of Granite Rock Mountain, That Son, an Giang Province, Vietnam},
  journal = {American Journal of Life Sciences},
  volume = {2},
  number = {5},
  pages = {282-291},
  doi = {10.11648/j.ajls.20140205.16},
  url = {https://doi.org/10.11648/j.ajls.20140205.16},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20140205.16},
  abstract = {Two hundred and thirty-seven bacterial isolates from 118 sample soils/weathered granite rock of That Son Mountain, An Giang province, Vietnam were isolated on Aleksandrov medium. Their colonies were round or irregular, white to yellow and their shape was rod, motile. Thirty four of two hundred and thirty-seven bacterial isolates were capable of dissolving both phosphate and potassium and seven strains had high phosphate and potassium dissolution capacity (>15 mg l-1 P2O5 and >10 mg l-1 K2O). These thirty four strains were identified by using PCR technique with specific primers fd1 and rP and DNA sequencing. The results of DNAsequencing were compared with GenBank database of NCBI by BLAST N software. The sequences from selected phosphate and potassium isolates (34 isolates) showed high degrees of similarity to those of the GenBank references (between 99% and 100%). Among these sequenced isolates, 8 isolates belong to Bacilli (23.53%) and 26 isolates belong to Proteobacteria (76.47%) including 21 alpha-proteobacteria (61.76%) and 5 gamma-proteobacteria (14.71%). Based on Pi value (nucleotide diversity), Proteobacteria group had the highest Theta values and Theta values (per sequence) from S of SNP for DNA polymorphism were calculated for each group and AlphaProteobacteria group had the highest values in comparistion with two groups. These results showed that 3 strains including CA09 (Agrobacterium tumefaciens), CA29 (Rhizobium tropici) and K16B (Azotobacter tropicalis) proposed as potential microbial inoculants or biofertilizers for sustainable crop production in sandy acid soil in Vietnam because of their benefits and biosafety.},
 year = {2014}
}

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TY  - JOUR
T1  - Isolation, Characterization and Identification of Phosphate- and Potassium- Solubilizing Bacteria from Weathered Materials of Granite Rock Mountain, That Son, an Giang Province, Vietnam
AU  - Nguyen Thi Don
AU  - Cao Ngoc Diep
Y1  - 2014/10/30
PY  - 2014
N1  - https://doi.org/10.11648/j.ajls.20140205.16
DO  - 10.11648/j.ajls.20140205.16
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 282
EP  - 291
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.20140205.16
AB  - Two hundred and thirty-seven bacterial isolates from 118 sample soils/weathered granite rock of That Son Mountain, An Giang province, Vietnam were isolated on Aleksandrov medium. Their colonies were round or irregular, white to yellow and their shape was rod, motile. Thirty four of two hundred and thirty-seven bacterial isolates were capable of dissolving both phosphate and potassium and seven strains had high phosphate and potassium dissolution capacity (>15 mg l-1 P2O5 and >10 mg l-1 K2O). These thirty four strains were identified by using PCR technique with specific primers fd1 and rP and DNA sequencing. The results of DNAsequencing were compared with GenBank database of NCBI by BLAST N software. The sequences from selected phosphate and potassium isolates (34 isolates) showed high degrees of similarity to those of the GenBank references (between 99% and 100%). Among these sequenced isolates, 8 isolates belong to Bacilli (23.53%) and 26 isolates belong to Proteobacteria (76.47%) including 21 alpha-proteobacteria (61.76%) and 5 gamma-proteobacteria (14.71%). Based on Pi value (nucleotide diversity), Proteobacteria group had the highest Theta values and Theta values (per sequence) from S of SNP for DNA polymorphism were calculated for each group and AlphaProteobacteria group had the highest values in comparistion with two groups. These results showed that 3 strains including CA09 (Agrobacterium tumefaciens), CA29 (Rhizobium tropici) and K16B (Azotobacter tropicalis) proposed as potential microbial inoculants or biofertilizers for sustainable crop production in sandy acid soil in Vietnam because of their benefits and biosafety.
VL  - 2
IS  - 5
ER  -

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