Determination of Amino Acids and Nutritional Aspects in Transgenic Soybean Exposed to Glyphosate

André Luiz de Souza Lacerda; Salete Aparecida Sagaziol; Daiana Schmidt; Ricardo Antunes Azevedo

doi:doi:10.11648/j.ajpb.20190403.14

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Determination of Amino Acids and Nutritional Aspects in Transgenic Soybean Exposed to Glyphosate

André Luiz de Souza Lacerda, Salete Aparecida Sagaziol, Daiana Schmidt, Ricardo Antunes Azevedo

Published in American Journal of Plant Biology (Volume 4, Issue 3)

Received: 15 September 2019 Accepted: 29 September 2019 Published: 11 October 2019

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Abstract

With the increase of glyphosate tolerant transgenic soybean cultivation areas, this substance has become the main herbicide for this crop, its use may interfere with the metabolism and nutritional aspects of transgenic plants. In this scenario, the objective of this work was to verify the oil, protein, phytate and amino acid concentrations in glyphosate tolerant soybean cultivar. For this, a randomized block experiment with 6 treatments and 4 repetitions was performed. The analyzed cultivar was genetically modified soybean BRS Valiosa RR. The treatments consisted of glyphosate applied once, that is, respectively in the doses: 1,5 and 2,0 L ha-1 of the commercial product, glyphosate applied sequentially in the doses: 1,5 / 1,5; 2.0 / 1.5 and 2.0 / 1.5 / 1.5 L ha^-1 of commercial products, with intervals of 15 to 20 days between applications and control (clean) weeded (without glyphosate application)). Grains were harvested, macerated and 200 mg of each sample was used for extraction of soluble amino acids. After extraction, triplicate aliquots were analyzed to determine total soluble amino acids. For quantitative determination of free amino acids, reverse phase HPLC was used after derivatization with o-ophydialdehyde (OPA) on a Spherisorb ODS-2 (C18) column. OPA derivatives were detected by fluorescence and protein contents were determined by the Kjeldhal and Soxhlet method. The data obtained were submitted to analysis of variance and F test of the statistical program ASSISTAT version 7.7 beta. Significant analyzes were performed by comparing means by Tukey test at 5% probability. In conclusion, the data indicate that although the transgenic event affects some routes, amino acid synthesis was not affected nor in nutritional terms at the glyphosate doses studied.

Published in	American Journal of Plant Biology (Volume 4, Issue 3)
DOI	10.11648/j.ajpb.20190403.14
Page(s)	40-45
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Soybean, Phytate, Amino Acids, Transgenics, Glyphosate

References

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André Luiz de Souza Lacerda, Salete Aparecida Sagaziol, Daiana Schmidt, Ricardo Antunes Azevedo. (2019). Determination of Amino Acids and Nutritional Aspects in Transgenic Soybean Exposed to Glyphosate. American Journal of Plant Biology, 4(3), 40-45. https://doi.org/10.11648/j.ajpb.20190403.14

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ACS Style

André Luiz de Souza Lacerda; Salete Aparecida Sagaziol; Daiana Schmidt; Ricardo Antunes Azevedo. Determination of Amino Acids and Nutritional Aspects in Transgenic Soybean Exposed to Glyphosate. Am. J. Plant Biol. 2019, 4(3), 40-45. doi: 10.11648/j.ajpb.20190403.14

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AMA Style

André Luiz de Souza Lacerda, Salete Aparecida Sagaziol, Daiana Schmidt, Ricardo Antunes Azevedo. Determination of Amino Acids and Nutritional Aspects in Transgenic Soybean Exposed to Glyphosate. Am J Plant Biol. 2019;4(3):40-45. doi: 10.11648/j.ajpb.20190403.14

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@article{10.11648/j.ajpb.20190403.14,
  author = {André Luiz de Souza Lacerda and Salete Aparecida Sagaziol and Daiana Schmidt and Ricardo Antunes Azevedo},
  title = {Determination of Amino Acids and Nutritional Aspects in Transgenic Soybean Exposed to Glyphosate},
  journal = {American Journal of Plant Biology},
  volume = {4},
  number = {3},
  pages = {40-45},
  doi = {10.11648/j.ajpb.20190403.14},
  url = {https://doi.org/10.11648/j.ajpb.20190403.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajpb.20190403.14},
  abstract = {With the increase of glyphosate tolerant transgenic soybean cultivation areas, this substance has become the main herbicide for this crop, its use may interfere with the metabolism and nutritional aspects of transgenic plants. In this scenario, the objective of this work was to verify the oil, protein, phytate and amino acid concentrations in glyphosate tolerant soybean cultivar. For this, a randomized block experiment with 6 treatments and 4 repetitions was performed. The analyzed cultivar was genetically modified soybean BRS Valiosa RR. The treatments consisted of glyphosate applied once, that is, respectively in the doses: 1,5 and 2,0 L ha-1 of the commercial product, glyphosate applied sequentially in the doses: 1,5 / 1,5; 2.0 / 1.5 and 2.0 / 1.5 / 1.5 L ha-1 of commercial products, with intervals of 15 to 20 days between applications and control (clean) weeded (without glyphosate application)). Grains were harvested, macerated and 200 mg of each sample was used for extraction of soluble amino acids. After extraction, triplicate aliquots were analyzed to determine total soluble amino acids. For quantitative determination of free amino acids, reverse phase HPLC was used after derivatization with o-ophydialdehyde (OPA) on a Spherisorb ODS-2 (C18) column. OPA derivatives were detected by fluorescence and protein contents were determined by the Kjeldhal and Soxhlet method. The data obtained were submitted to analysis of variance and F test of the statistical program ASSISTAT version 7.7 beta. Significant analyzes were performed by comparing means by Tukey test at 5% probability. In conclusion, the data indicate that although the transgenic event affects some routes, amino acid synthesis was not affected nor in nutritional terms at the glyphosate doses studied.},
 year = {2019}
}

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TY - JOUR
T1 - Determination of Amino Acids and Nutritional Aspects in Transgenic Soybean Exposed to Glyphosate
AU - André Luiz de Souza Lacerda
AU - Salete Aparecida Sagaziol
AU - Daiana Schmidt
AU - Ricardo Antunes Azevedo
Y1 - 2019/10/11
PY - 2019
N1 - https://doi.org/10.11648/j.ajpb.20190403.14
DO - 10.11648/j.ajpb.20190403.14
T2 - American Journal of Plant Biology
JF - American Journal of Plant Biology
JO - American Journal of Plant Biology
SP - 40
EP - 45
PB - Science Publishing Group
SN - 2578-8337
UR - https://doi.org/10.11648/j.ajpb.20190403.14
AB - With the increase of glyphosate tolerant transgenic soybean cultivation areas, this substance has become the main herbicide for this crop, its use may interfere with the metabolism and nutritional aspects of transgenic plants. In this scenario, the objective of this work was to verify the oil, protein, phytate and amino acid concentrations in glyphosate tolerant soybean cultivar. For this, a randomized block experiment with 6 treatments and 4 repetitions was performed. The analyzed cultivar was genetically modified soybean BRS Valiosa RR. The treatments consisted of glyphosate applied once, that is, respectively in the doses: 1,5 and 2,0 L ha-1 of the commercial product, glyphosate applied sequentially in the doses: 1,5 / 1,5; 2.0 / 1.5 and 2.0 / 1.5 / 1.5 L ha-1 of commercial products, with intervals of 15 to 20 days between applications and control (clean) weeded (without glyphosate application)). Grains were harvested, macerated and 200 mg of each sample was used for extraction of soluble amino acids. After extraction, triplicate aliquots were analyzed to determine total soluble amino acids. For quantitative determination of free amino acids, reverse phase HPLC was used after derivatization with o-ophydialdehyde (OPA) on a Spherisorb ODS-2 (C18) column. OPA derivatives were detected by fluorescence and protein contents were determined by the Kjeldhal and Soxhlet method. The data obtained were submitted to analysis of variance and F test of the statistical program ASSISTAT version 7.7 beta. Significant analyzes were performed by comparing means by Tukey test at 5% probability. In conclusion, the data indicate that although the transgenic event affects some routes, amino acid synthesis was not affected nor in nutritional terms at the glyphosate doses studied.
VL - 4
IS - 3
ER -

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Author Information

André Luiz de Souza Lacerda

Department of Environment, City Hall of Valinhos, Valinhos, Brazil
Salete Aparecida Sagaziol

Department of Genetic, S?o Paulo of University, Piracicaba, Brazil
Daiana Schmidt

Department of Genetic, S?o Paulo of University, Piracicaba, Brazil
Ricardo Antunes Azevedo

Department of Genetic, S?o Paulo of University, Piracicaba, Brazil

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APA Style

André Luiz de Souza Lacerda, Salete Aparecida Sagaziol, Daiana Schmidt, Ricardo Antunes Azevedo. (2019). Determination of Amino Acids and Nutritional Aspects in Transgenic Soybean Exposed to Glyphosate. American Journal of Plant Biology, 4(3), 40-45. https://doi.org/10.11648/j.ajpb.20190403.14

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ACS Style

André Luiz de Souza Lacerda; Salete Aparecida Sagaziol; Daiana Schmidt; Ricardo Antunes Azevedo. Determination of Amino Acids and Nutritional Aspects in Transgenic Soybean Exposed to Glyphosate. Am. J. Plant Biol. 2019, 4(3), 40-45. doi: 10.11648/j.ajpb.20190403.14

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AMA Style

André Luiz de Souza Lacerda, Salete Aparecida Sagaziol, Daiana Schmidt, Ricardo Antunes Azevedo. Determination of Amino Acids and Nutritional Aspects in Transgenic Soybean Exposed to Glyphosate. Am J Plant Biol. 2019;4(3):40-45. doi: 10.11648/j.ajpb.20190403.14

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@article{10.11648/j.ajpb.20190403.14,
  author = {André Luiz de Souza Lacerda and Salete Aparecida Sagaziol and Daiana Schmidt and Ricardo Antunes Azevedo},
  title = {Determination of Amino Acids and Nutritional Aspects in Transgenic Soybean Exposed to Glyphosate},
  journal = {American Journal of Plant Biology},
  volume = {4},
  number = {3},
  pages = {40-45},
  doi = {10.11648/j.ajpb.20190403.14},
  url = {https://doi.org/10.11648/j.ajpb.20190403.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajpb.20190403.14},
  abstract = {With the increase of glyphosate tolerant transgenic soybean cultivation areas, this substance has become the main herbicide for this crop, its use may interfere with the metabolism and nutritional aspects of transgenic plants. In this scenario, the objective of this work was to verify the oil, protein, phytate and amino acid concentrations in glyphosate tolerant soybean cultivar. For this, a randomized block experiment with 6 treatments and 4 repetitions was performed. The analyzed cultivar was genetically modified soybean BRS Valiosa RR. The treatments consisted of glyphosate applied once, that is, respectively in the doses: 1,5 and 2,0 L ha-1 of the commercial product, glyphosate applied sequentially in the doses: 1,5 / 1,5; 2.0 / 1.5 and 2.0 / 1.5 / 1.5 L ha-1 of commercial products, with intervals of 15 to 20 days between applications and control (clean) weeded (without glyphosate application)). Grains were harvested, macerated and 200 mg of each sample was used for extraction of soluble amino acids. After extraction, triplicate aliquots were analyzed to determine total soluble amino acids. For quantitative determination of free amino acids, reverse phase HPLC was used after derivatization with o-ophydialdehyde (OPA) on a Spherisorb ODS-2 (C18) column. OPA derivatives were detected by fluorescence and protein contents were determined by the Kjeldhal and Soxhlet method. The data obtained were submitted to analysis of variance and F test of the statistical program ASSISTAT version 7.7 beta. Significant analyzes were performed by comparing means by Tukey test at 5% probability. In conclusion, the data indicate that although the transgenic event affects some routes, amino acid synthesis was not affected nor in nutritional terms at the glyphosate doses studied.},
 year = {2019}
}

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