Determination of in Vitro Antiviral Activity of Nerium Oleander Distillate against to Parainfluenza-3 Virus

Oguzhan Avci; Burak Dik

doi:doi:10.11648/j.avs.20140205.14

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Determination of in Vitro Antiviral Activity of Nerium Oleander Distillate against to Parainfluenza-3 Virus

Oguzhan Avci, Burak Dik

Published in Animal and Veterinary Sciences (Volume 2, Issue 5)

Received: 27 August 2014 Accepted: 5 September 2014 Published: 20 September 2014

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Abstract

Chemical extract of Nerium oleander (NO) is identified as in vitro antibacterial and antifungal activities. However, there is no any study about antiviral effect of NO distillate (NOD). The aim of this study was to evaluate in vitro effects of NOD alone and combined with acyclovir on Parainfluenza-3 (PI-3) virus. Lyophilized NOD was dissolved at concentration of 10 mg/mL with distillated water and filtered. The thiazolyl blue test was used to evaluate the cytotoxic activity of the NOD, and acyclovir. NOD did not cause cytotoxicity against to MDBK cells up to 10 mg/mL concentrations while acyclovir did not cause cytotoxicity up to 0.97 µg/mL. NOD (10 mg/mL), acyclovir (0.97 µg/mL) and NOD + acyclovir were treated with 100 TCID50 diluted PI-3 in 96-well plates. Antiviral activity of NOD was statistically (P<0.05) different from NOD + acyclovir against PI-3. The most effective results obtained from using single of NOD (P<0.05) while acyclovir was not effective (P>0.05). In conclusion, it is referred that NOD has antiviral effect and it can be evaluated in the treatment of PI-3 in future.

Published in	Animal and Veterinary Sciences (Volume 2, Issue 5)
DOI	10.11648/j.avs.20140205.14
Page(s)	150-153
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

PI-3, Nerium Oleander, Acyclovir, Antiviral

References

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APA Style

Oguzhan Avci, Burak Dik. (2014). Determination of in Vitro Antiviral Activity of Nerium Oleander Distillate against to Parainfluenza-3 Virus. Animal and Veterinary Sciences, 2(5), 150-153. https://doi.org/10.11648/j.avs.20140205.14

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ACS Style

Oguzhan Avci; Burak Dik. Determination of in Vitro Antiviral Activity of Nerium Oleander Distillate against to Parainfluenza-3 Virus. Anim. Vet. Sci. 2014, 2(5), 150-153. doi: 10.11648/j.avs.20140205.14

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AMA Style

Oguzhan Avci, Burak Dik. Determination of in Vitro Antiviral Activity of Nerium Oleander Distillate against to Parainfluenza-3 Virus. Anim Vet Sci. 2014;2(5):150-153. doi: 10.11648/j.avs.20140205.14

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@article{10.11648/j.avs.20140205.14,
  author = {Oguzhan Avci and Burak Dik},
  title = {Determination of in Vitro Antiviral Activity of Nerium Oleander Distillate against to Parainfluenza-3 Virus},
  journal = {Animal and Veterinary Sciences},
  volume = {2},
  number = {5},
  pages = {150-153},
  doi = {10.11648/j.avs.20140205.14},
  url = {https://doi.org/10.11648/j.avs.20140205.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20140205.14},
  abstract = {Chemical extract of Nerium oleander (NO) is identified as in vitro antibacterial and antifungal activities. However, there is no any study about antiviral effect of NO distillate (NOD). The aim of this study was to evaluate in vitro effects of NOD alone and combined with acyclovir on Parainfluenza-3 (PI-3) virus. Lyophilized NOD was dissolved at concentration of 10 mg/mL with distillated water and filtered. The thiazolyl blue test was used to evaluate the cytotoxic activity of the NOD, and acyclovir. NOD did not cause cytotoxicity against to MDBK cells up to 10 mg/mL concentrations while acyclovir did not cause cytotoxicity up to 0.97 µg/mL. NOD (10 mg/mL), acyclovir (0.97 µg/mL) and NOD + acyclovir were treated with 100 TCID50 diluted PI-3 in 96-well plates. Antiviral activity of NOD was statistically (P0.05). In conclusion, it is referred that NOD has antiviral effect and it can be evaluated in the treatment of PI-3 in future.},
 year = {2014}
}

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TY  - JOUR
T1  - Determination of in Vitro Antiviral Activity of Nerium Oleander Distillate against to Parainfluenza-3 Virus
AU  - Oguzhan Avci
AU  - Burak Dik
Y1  - 2014/09/20
PY  - 2014
N1  - https://doi.org/10.11648/j.avs.20140205.14
DO  - 10.11648/j.avs.20140205.14
T2  - Animal and Veterinary Sciences
JF  - Animal and Veterinary Sciences
JO  - Animal and Veterinary Sciences
SP  - 150
EP  - 153
PB  - Science Publishing Group
SN  - 2328-5850
UR  - https://doi.org/10.11648/j.avs.20140205.14
AB  - Chemical extract of Nerium oleander (NO) is identified as in vitro antibacterial and antifungal activities. However, there is no any study about antiviral effect of NO distillate (NOD). The aim of this study was to evaluate in vitro effects of NOD alone and combined with acyclovir on Parainfluenza-3 (PI-3) virus. Lyophilized NOD was dissolved at concentration of 10 mg/mL with distillated water and filtered. The thiazolyl blue test was used to evaluate the cytotoxic activity of the NOD, and acyclovir. NOD did not cause cytotoxicity against to MDBK cells up to 10 mg/mL concentrations while acyclovir did not cause cytotoxicity up to 0.97 µg/mL. NOD (10 mg/mL), acyclovir (0.97 µg/mL) and NOD + acyclovir were treated with 100 TCID50 diluted PI-3 in 96-well plates. Antiviral activity of NOD was statistically (P0.05). In conclusion, it is referred that NOD has antiviral effect and it can be evaluated in the treatment of PI-3 in future.
VL  - 2
IS  - 5
ER  -

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Author Information

Oguzhan Avci

Department of Virology, Faculty of Veterinary Medicine, University of Selcuk, Konya, Turkey
Burak Dik

Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Selcuk, Konya, Turkey

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Plain Text BibTeX RIS

APA Style

Oguzhan Avci, Burak Dik. (2014). Determination of in Vitro Antiviral Activity of Nerium Oleander Distillate against to Parainfluenza-3 Virus. Animal and Veterinary Sciences, 2(5), 150-153. https://doi.org/10.11648/j.avs.20140205.14

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ACS Style

Oguzhan Avci; Burak Dik. Determination of in Vitro Antiviral Activity of Nerium Oleander Distillate against to Parainfluenza-3 Virus. Anim. Vet. Sci. 2014, 2(5), 150-153. doi: 10.11648/j.avs.20140205.14

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AMA Style

Oguzhan Avci, Burak Dik. Determination of in Vitro Antiviral Activity of Nerium Oleander Distillate against to Parainfluenza-3 Virus. Anim Vet Sci. 2014;2(5):150-153. doi: 10.11648/j.avs.20140205.14

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@article{10.11648/j.avs.20140205.14,
  author = {Oguzhan Avci and Burak Dik},
  title = {Determination of in Vitro Antiviral Activity of Nerium Oleander Distillate against to Parainfluenza-3 Virus},
  journal = {Animal and Veterinary Sciences},
  volume = {2},
  number = {5},
  pages = {150-153},
  doi = {10.11648/j.avs.20140205.14},
  url = {https://doi.org/10.11648/j.avs.20140205.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20140205.14},
  abstract = {Chemical extract of Nerium oleander (NO) is identified as in vitro antibacterial and antifungal activities. However, there is no any study about antiviral effect of NO distillate (NOD). The aim of this study was to evaluate in vitro effects of NOD alone and combined with acyclovir on Parainfluenza-3 (PI-3) virus. Lyophilized NOD was dissolved at concentration of 10 mg/mL with distillated water and filtered. The thiazolyl blue test was used to evaluate the cytotoxic activity of the NOD, and acyclovir. NOD did not cause cytotoxicity against to MDBK cells up to 10 mg/mL concentrations while acyclovir did not cause cytotoxicity up to 0.97 µg/mL. NOD (10 mg/mL), acyclovir (0.97 µg/mL) and NOD + acyclovir were treated with 100 TCID50 diluted PI-3 in 96-well plates. Antiviral activity of NOD was statistically (P0.05). In conclusion, it is referred that NOD has antiviral effect and it can be evaluated in the treatment of PI-3 in future.},
 year = {2014}
}

Copy | Download

TY  - JOUR
T1  - Determination of in Vitro Antiviral Activity of Nerium Oleander Distillate against to Parainfluenza-3 Virus
AU  - Oguzhan Avci
AU  - Burak Dik
Y1  - 2014/09/20
PY  - 2014
N1  - https://doi.org/10.11648/j.avs.20140205.14
DO  - 10.11648/j.avs.20140205.14
T2  - Animal and Veterinary Sciences
JF  - Animal and Veterinary Sciences
JO  - Animal and Veterinary Sciences
SP  - 150
EP  - 153
PB  - Science Publishing Group
SN  - 2328-5850
UR  - https://doi.org/10.11648/j.avs.20140205.14
AB  - Chemical extract of Nerium oleander (NO) is identified as in vitro antibacterial and antifungal activities. However, there is no any study about antiviral effect of NO distillate (NOD). The aim of this study was to evaluate in vitro effects of NOD alone and combined with acyclovir on Parainfluenza-3 (PI-3) virus. Lyophilized NOD was dissolved at concentration of 10 mg/mL with distillated water and filtered. The thiazolyl blue test was used to evaluate the cytotoxic activity of the NOD, and acyclovir. NOD did not cause cytotoxicity against to MDBK cells up to 10 mg/mL concentrations while acyclovir did not cause cytotoxicity up to 0.97 µg/mL. NOD (10 mg/mL), acyclovir (0.97 µg/mL) and NOD + acyclovir were treated with 100 TCID50 diluted PI-3 in 96-well plates. Antiviral activity of NOD was statistically (P0.05). In conclusion, it is referred that NOD has antiviral effect and it can be evaluated in the treatment of PI-3 in future.
VL  - 2
IS  - 5
ER  -

Copy | Download