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Phenolic Compounds of Napek Leave (Zizyphus Spina-Christi L.) as Natural Antioxidants

Received: 25 July 2014     Accepted: 11 August 2014     Published: 20 August 2014
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Abstract

Crude juices of Sidr (Zizyphus Spina-Christi L.) from leaves were obtained by hydraulic press. The levels of polyphenolic compounds in the (leaves) juice were 510.00 and 722.00ppm. Aliquots of the concentrated sidr juice (leaves), represent 200, 400, 800 and 1600ppm and butylated hydroxyl toluene (BHT, 200ppm) were investigated by Rancimat method at 100ºC and 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging method. These compounds were administrated to rats daily for 6 weeks by stomach tube. The liver (Aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase activities) and kidney (bilirubin, uric acid and creatinine) function tests and serum contents (total lipids, total cholesterol and low and high-density lipoproteins) were measured to assess the safety limits of the phenolic compounds in the sidr juice (fruits and leaves). The data of the aforementioned measurements indicates that the administration of sidr juice (leaves) did not cause any changes in liver and kidney functions. On the contrary, BHT at 200ppm induced significant increases in the enzyme activities and the serum levels of total lipids, uric acid and creatinine.

Published in Journal of Food and Nutrition Sciences (Volume 2, Issue 5)
DOI 10.11648/j.jfns.20140205.11
Page(s) 207-214
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Zizyphus Spina- Christi L., Phenolic Compounds, Antioxidants, Phytochemistry

References
[1] Pong K, (2003). Oxidative stress in neurodegenerative diseases: Therapeutic implications for superoxide dismutase mimetics. Expert Opinion in Biological Therapy, 3: 127-139.
[2] Bandyopodhyay M, Chakraborty R and Raychaudlhuri U (2007). A process for preparing a natural antioxidant enriched diary product. LWT-Food Science & Technology, 40: 842-851.
[3] Seyuum A, Asres K and El-Fiky F K (2006). Structure-radical scavenging activity relationships of flavonoids. Phytochemistry, 67: 2058-2070.
[4] Ammar R B, Bhour W, Sghaier M B et al. (2009). Antioxidant and free radical-scavenging properties of three flavonoids isolated from the leaves of Rhamnus alaternus L.: A structure-activity relationship study. Food Chemistry, 116: 258-264.
[5] Kahkonen M P, Hopia A I, Vuorela H J, et al. (1999). Antioxidant activity of plant extracts containing phenolic compounds. Journal of Agricultural & Food Chemistry, 47: 3954-3962.
[6] Willams R J, Spencer J P and Rice-Evans C (2004). Flavonoids: Antioxidants or signaling molecular, Free Radical Biology & Medicine, 36: 838-849.
[7] Ross J A and Kasum C M (2002). Dietary flavonoids: bioavailability, metabolic effects, and safety. Annu. Rev. Nutr. 22: 19-34.
[8] Bors W, Michel C and Stettmaier K (2001). Structure-activity relationships governing antioxidant capacities of plant polyphenols. Methods in Enzymology, 335: 166-180.
[9] Cai Y Z, Sun M and Xing J, et al. (2006). Structure-radical scavenging activity relationships of phenolic compounds from traditional Chinese medicine plants. Life Science, 78: 2872-2888.
[10] Branen A, (1975). Toxicology and biochemistry of butylated hydroxanisole and butylated hydroxytoluene. Journal of the American Oil Chemical Society, 52: 59-63.
[11] Youssef H E, Khedr A A. Mahran M Z (2011). Hepatoprotective activity and antioxidant effects of Napk (Zizyphus spina-christi L.) fruits on rats hepatoxicity induced by carbon tetrachloride. Nutrition Science, 9: 1-7.
[12] Zargari A (1988). Medicinal plants. Tehran University Press, Ira.2: 42-45.
[13] Adzu B and Haruna A K (2007). Studied on the use of Zizyphus spina-christi L. against pain in rats and mice. African Journal of Biotechnology, 6: 1317-1324.
[14] Abalaka M E, Daniyan S Y and Mann A (2010). Evaluation of the antimicrobial activities of two Ziziphus species (Ziziphus mauritiana L. and Ziziphus spina-christi L.) on some microbial pathogens. Afr. J. Pharm. Pharmacol. 4(4):135-139.
[15] Han B H and Park M H (1986). Folk medicine: The art and science. The American Chemical Society, Washington DC, P.205.
[16] Kirtikar K R and Basu B D (1984). Indian medicinal plants, Lalit Mohan Basu, Allahabad, P.593.
[17] Adzu B, Amos S, Wambebe C et al. (2001) Antinociceptive activity of Zizyphus spina-christi root bark extract. Fitoterapia, 72: 334-350.
[18] Kaaria I (1988). Seed production, dispersal and germination in cryptostegia grandifolia and zizphus mauritiana, two invasive shrubs in tropical woodlands of Northern Australia. Australia Journal Ecology, 21: 324-331
[19] Michel A (2002). Tree, shrub and liana of West African zone. Margraf Publishers GMBH, Paris, P. 440.
[20] Abdel-Wahhab M A, Omar E A, Abdel-Galil, M M et al. (2007). Zizyphus spina-christi L. extract protect against aflatoxin BI initiated hepatic carcinogenicity. African Journal Traditional, Complementary & Alternative Medicines, 4: 248-256
[21] Adzu B, Amos S, Amiza M B et al. (2003). Evaluation of the antidiarrhoeal effects of Zizyphus spina-christi L. stem bark in rats. Acta Tropica, 87: 245-250.
[22] Asgarpanah J and Haghighat E (2012). Phytochemistry and pharmacologic properties of Zizyphus spina-christi L. willd. African Journal of Pharmacy & Pharmacology, 6: 2332-2339.
[23] Nazif M N (2002). Phytoconstituents of Zizyphus spina-christi L. fruits and their antimicrobial activity, Food Chemistry, 76: 77-81.
[24] El-kamali, H H and Mahioub S A (2009). Antibacterial activities of francoeuria crispa, pulicaria undulate, Zizyphus spina-christi and cucurbita pepo against seven standard pathogenic bacteria. Ethnobotany, Leaflets, 13: 722-733.
[25] Alsaimary I E (2012). A study of antibacterial activity of plant extracts on bacterial pathogens isolated from eye infections. International Journal Microbiology. 4: 1-5.
[26] Korji S H A (2012). Inhibition of nitrate reductase production from Gram-negative bacteria using Ziziphus spina-christi extract and comparing with some antibiotics. Iraqi J. Agric. Sci. 43(2):144-150.
[27] Rhee M H, Park H J, Cho J Y (2009). Salicornia herbaceae: Botanical, Chemical and pharmacological review of halophyte marsh plant. J. Med. Plants Res. 3(8):548-555.
[28] Gutfinger T (1981). Polyphenols in olive virgin oils, Journal American Oil Chemical Society, 58: 996-998.
[29] Mateos R, Espartero J L, Trujillo M et al. (2001). Determination of phenols, flavones and lignanes of virgin olive oils by solid phase extraction and high performance liquid chromatography with diode array ultraviolet detection. Journal Agricultural Food Chemistry, 49: 2185-2192.
[30] Gutierrez, F. (1989): Determination de la estabilidade oxidative de acuities de olive virgrnes. Comparacion entre del metodo A. O. M. Y. el metodo Rancimat. Grasas Y Aceities, 40: 1-5.
[31] Cheel C J, Theoduloz C, Rodriguez J A et al. (2007). Free-radical scavenging activity and phenolic content in achenes and thalamus from Frgaria chilonsis ssp. Food Chemistry, 102: 36-44.
[32] A O A C (2005). Official Methods of Analysis of the Association of Official Analytical Chemists, 18th edn (edited by W. Horwitz). Pp. 62. Gaithersburg, MO: AOAC.
[33] Schermer S. (1967). The blood morphology of laboratory animals. Pp. 350. Philadelphia: FA, Davis Co.
[34] Kachmar J F. and Moss, D W. (1976). Enzymes. In: Fundamentals of Clinical Chemistry (edited by N. Tiez). Pp. 666-672. Philadelphia, PA: W. B. Saunders Co.
[35] Bergmryer H U and Harder M (1986). A colorimetric method for determination of serum glutamic oxaloacetic and glutamic pyruvic transaminase. Clinical Biochemistry, 24: 28-34.
[36] Varley H, Gewnlock A and Bell M (1980). Practical clinical biochemistry, Vol. 1, 5th edn. Pp. 741, 897. London: Williams Heinemen Medical Books, Ltd.
[37] Fawcett J K, and Scott J E (1960). Enzymatic, colorimetric method for determination urea in serum, plasma and urine. Journal of clinical Pathology, 13: 156-162.
[38] Dounas B T, Perry B and Jenderze-Jczak L (1981). Colorimetric method for determination total bilirubin, direct and indirect bilirubin. Clinical Chemistry, 33: 1349-1356.
[39] Barham D and Trinder P. (1972). Enzymatic colorimetric method for determination uric acid in serum plasma and urine. Analyst, 97: 142-146.
[40] Roechlau P, Bernt E and Gruber W L (1974). Kinetics of the cholesterol sulfuric acid reaction. A fast kinetic method for serum cholesterol. Clinical Chemistry & Clinical Biochemistry, 12: 403-408.
[41] Assmann G (1979). Cholesterol determination in high density lipoproteins separated by three different methods. Internist, 20: 559-604.
[42] Frings C S and Dunn R T (1979). Colorimetric method for determination total serum lipids based on the sulphopospho vanillin reaction. Am. J. Clin. Pathol. 53: 89-91.
[43] Allen J C and Hamilton R J (1983). Rancidity in foods. Pp. 85-173. London and NewYork: Applied Sinece Publishers.
[44] Baron D N (1987). A short textbook of chemical pathology, 4th edn. Pp. 188-228. Kent, Great Britain: English Langgoage book Society Hadder & Stoghton Ltd.
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  • APA Style

    Maliha. A. AL-Marzooq. (2014). Phenolic Compounds of Napek Leave (Zizyphus Spina-Christi L.) as Natural Antioxidants. Journal of Food and Nutrition Sciences, 2(5), 207-214. https://doi.org/10.11648/j.jfns.20140205.11

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    ACS Style

    Maliha. A. AL-Marzooq. Phenolic Compounds of Napek Leave (Zizyphus Spina-Christi L.) as Natural Antioxidants. J. Food Nutr. Sci. 2014, 2(5), 207-214. doi: 10.11648/j.jfns.20140205.11

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    AMA Style

    Maliha. A. AL-Marzooq. Phenolic Compounds of Napek Leave (Zizyphus Spina-Christi L.) as Natural Antioxidants. J Food Nutr Sci. 2014;2(5):207-214. doi: 10.11648/j.jfns.20140205.11

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  • @article{10.11648/j.jfns.20140205.11,
      author = {Maliha. A. AL-Marzooq},
      title = {Phenolic Compounds of Napek Leave (Zizyphus Spina-Christi L.) as Natural Antioxidants},
      journal = {Journal of Food and Nutrition Sciences},
      volume = {2},
      number = {5},
      pages = {207-214},
      doi = {10.11648/j.jfns.20140205.11},
      url = {https://doi.org/10.11648/j.jfns.20140205.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jfns.20140205.11},
      abstract = {Crude juices of Sidr (Zizyphus Spina-Christi L.) from leaves were obtained by hydraulic press. The levels of polyphenolic compounds in the (leaves) juice were 510.00 and 722.00ppm. Aliquots of the concentrated sidr juice (leaves), represent 200, 400, 800 and 1600ppm and butylated hydroxyl toluene (BHT, 200ppm) were investigated by Rancimat method at 100ºC and 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging method. These compounds were administrated to rats daily for 6 weeks by stomach tube. The liver (Aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase activities) and kidney (bilirubin, uric acid and creatinine) function tests and serum contents (total lipids, total cholesterol and low and high-density lipoproteins) were measured to assess the safety limits of the phenolic compounds in the sidr juice (fruits and leaves). The data of the aforementioned measurements indicates that the administration of sidr juice (leaves) did not cause any changes in liver and kidney functions. On the contrary, BHT at 200ppm induced significant increases in the enzyme activities and the serum levels of total lipids, uric acid and creatinine.},
     year = {2014}
    }
    

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    T1  - Phenolic Compounds of Napek Leave (Zizyphus Spina-Christi L.) as Natural Antioxidants
    AU  - Maliha. A. AL-Marzooq
    Y1  - 2014/08/20
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    N1  - https://doi.org/10.11648/j.jfns.20140205.11
    DO  - 10.11648/j.jfns.20140205.11
    T2  - Journal of Food and Nutrition Sciences
    JF  - Journal of Food and Nutrition Sciences
    JO  - Journal of Food and Nutrition Sciences
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    UR  - https://doi.org/10.11648/j.jfns.20140205.11
    AB  - Crude juices of Sidr (Zizyphus Spina-Christi L.) from leaves were obtained by hydraulic press. The levels of polyphenolic compounds in the (leaves) juice were 510.00 and 722.00ppm. Aliquots of the concentrated sidr juice (leaves), represent 200, 400, 800 and 1600ppm and butylated hydroxyl toluene (BHT, 200ppm) were investigated by Rancimat method at 100ºC and 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging method. These compounds were administrated to rats daily for 6 weeks by stomach tube. The liver (Aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase activities) and kidney (bilirubin, uric acid and creatinine) function tests and serum contents (total lipids, total cholesterol and low and high-density lipoproteins) were measured to assess the safety limits of the phenolic compounds in the sidr juice (fruits and leaves). The data of the aforementioned measurements indicates that the administration of sidr juice (leaves) did not cause any changes in liver and kidney functions. On the contrary, BHT at 200ppm induced significant increases in the enzyme activities and the serum levels of total lipids, uric acid and creatinine.
    VL  - 2
    IS  - 5
    ER  - 

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Author Information
  • Food Science & Nutrition Department, Faculty of Agriculture Science & Foods, King Faisal University, Saudi Arabia

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