Protective Effect of Aerial Parts of <i>Portulaca oleracea</i> and <i>Ficus carica</i> Leaves Against Diclofenac-Sodium Induced Hepatotoxicity in Rats

Mohamed Abd El-Ghany El-Sayed; Omayma El-Sayed Shaltot; Mokhtar Ibrahim Yousef; Entisar Abd El-Mohsen El-Difrawy

doi:doi:10.11648/j.jfns.20190701.11

| Peer-Reviewed

Protective Effect of Aerial Parts of Portulaca oleracea and Ficus carica Leaves Against Diclofenac-Sodium Induced Hepatotoxicity in Rats

Mohamed Abd El-Ghany El-Sayed, Omayma El-Sayed Shaltot, Mokhtar Ibrahim Yousef, Entisar Abd El-Mohsen El-Difrawy

Published in Journal of Food and Nutrition Sciences (Volume 7, Issue 1)

Received: 11 March 2019 Accepted: 26 April 2019 Published: 23 May 2019

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

The present experiment is aimed to evaluate the hepatoprotective effects of purslane (PuE) and fig leaves (FlE) extracts on diclofenac-sodium (DS) induced hepatotoxicity in rats. Adult male rats were pretreated orally with PuE and FlE extracts at a dose of 10ml/kg and 200mg/kg body weight, respectively for 14 days. Co-treatment of DS 16mg/kg body weight was given orally for 7days. The present results demonstrated that the treatment with PuE and FlE combination with DS induced a marked improvement in the studied parameters. Data indicated that there were no significant differences in relative liver weight (RLW) and relative kidney weight (RKW) in group treated with PuE and FlE and /or their combination as compared to control. Plasma liver enzyme activities as well as bilirubin levels were increased in the groups receiving diclofenac only or in combination as compared with control group. However, the administration of PuE and FlE ameliorated DS induced hepatotoxicity by improving antioxidant status, decreasing inflammation, lowering TBARS and weakening the adverse effect of diclofenac on hepatic tissues. Liver injury was confirmed by the histological changes. Taken together, the present study concluded that enhancement of antioxidants and promising activity against diclofenac-induced hepatotoxicity may a result for the effect of PuE and FlE.

Published in	Journal of Food and Nutrition Sciences (Volume 7, Issue 1)
DOI	10.11648/j.jfns.20190701.11
Page(s)	1-7
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Antioxidant Activity, Portulaca oleracea (Pursalne), Ficus carica (Fig Leaves), Diclofenac-Sodium, Hepatotoxicity

References

[1]	Subramonium, A., Pushpangada, P. (1999). Development of Phytomedicines for liver diseases. Indian J Pharmacology, 31, 166- 175.
[2]	Gagliano, N., Grizzi, F., & Annoni, G. (2007). Mechanism of aging and liver functions, Dig. Dis. Sci. 25, 118–123.
[3]	Dey, P., Saha, M. R., Sen, A. (2013). An overview on drug-induced hepatotoxicity. Asian J Pharm Clin Res, 6(4), 1–4.
[4]	Banks, A. T., Zimmerman, H. J., Ishak, K. G., & Harter, J. G. (1995). Diclofenac-associated hepatotoxicity: analysis of 180 cases reported to the Food and Drug Administration as adverse reactions. Hepatology 22, 820–827.
[5]	Franceschi, F., Saviano, L., Petruzziello, C., Gabrielli, M., Santarelli, L., Capaldi, L., Di Leo M., Migneco, A., Gilardi, E., Merra, G., & Ojetti, V. (2016). Safety and eﬃcacy of low doses of diclofenac on acute pain in the emergency setting. Eur. Rev. Med. Pharmacol. Sci, 20, 4401–4408.
[6]	Bessone, F. (2010). Non-steroidal anti-inﬂammatory drugs: what is the actual risk of liver damage? World J. Gastroenterol. 16, 5651–5661.
[7]	Gómez-Lechón, M. J., Ponsoda, X., O'Connor, E., Donato, T., Castell, J. V., & Jover. V. (2003). Diclofenac induces apoptosis in hepatocytes by alteration of mitochondrial function and generation of ROS, Biochem. Pharmacol. 66, 2155–2167.
[8]	Jain, S. P., Takade AR, Joshi UM, Kale RH, Purohit RN (2005) Protective effect of Gingko biloba on antitubercular drugs- induced hepatotoxicity in rats. Indian Drugs 42:167.
[9]	Hyam. R., Pankhurst, P. (1995). Plants and their names: A concise dictionary. Oxford: Oxford University Press, p. 545.
[10]	Gonnella, M., Charfeddine, M., Conversa, G., & Santamaria, P. (2010). Purslane: a review of its potential for health and agricultural aspects. Eur. J. Plant Sci. Biotechnol. 4, 131–136.
[11]	Zakaria, M. N. M., Islam, M. W., Radhakrishnan, R., Habibullah, M., Chan, K. (1998). Evaluation of anti-inflammatory activity of Portulaca species. J Pharmacy Pharm 50:227–231.
[12]	Al-Bishri, W. M., Abdel-Reheim, E. S., & Zaki, A. R. (2017). Purslane protects against the reproductive toxicity of carbamazepine treatment in pilocarpine-induced epilepsy model. Asian Pac J Trop Biomed; 7(4): 339–346.
[13]	El-Newary, S. A., (2016). The hypolipidemic effect of Portulaca oleracea L. stem on hyperlipidemic Wister Albino rats. Annals of Agricultural Science 61(1), 111– 124.
[14]	Manda, S. c., Mukherjee, K. P., Saha, K., Das, J., Pal, M., & Saha, P. B. (1997). Hypoglycaemic activity of Ficus carica L. leaves in streptozotoin-induced diabetic rats. Nat. Pro. Sci, 3(1), 38-41.
[15]	Vaya, J., & Mahmood, S. (2006). Flavonoid content in leaf extracts of the fig (Ficus carica L.), carob (Ceratonia siliqua L.) and pistachio (Pistacia lentiscus L.). BioFactors 28, 169–175.
[16]	Vikas, V. P., & Vijay, R. P. (2011). Ficus carica Linn. An overview. Res. J. Med. Plant 5, 246–253.
[17]	Perez, C., Dominguez, E., Canal, J. R. (2000). Hypoglycaemic activity of an aqueous extract from Ficus carica (fig tree) leaves in streptozotocin diabetic rats. Pharmac. Biol. 38, 181–186.
[18]	Gond, N. Y., & Khadabadi, S. S. (2008). Hepatoprotective activity of Ficus carica leaf extract on rifampicin-induced hepatic damage in rats. J. Pharm. Sci. 70, 364–372.
[19]	Mohamed, A. I., & Hussein, A. S. (1994). Chemical composition of purslane (Portulacaoleracea). Plant Foods for Human Nutrition 45, 1-9.
[20]	Dkhil, M. A., Abd el Moneim, A. E., Al-Quraishy, S., Saleh, R. A. (2011). Antioxidant effect of purslane (Portulaca oleracea) and its mechanism of action, J. Med. Plants Res. 5, 1589–1593.
[21]	Nebedum, J. O., Udeafor, P. C., & Okeke, C. U. (2010). Comparative effects of ethanolic extracts of Ficus carica and Mucuna pruriens leaves on haematological parameters in albino rats. Biokemistri (22), 2; 77-84.
[22]	Jollow, D. J., Michell, J. R., Zampaglionic, & Gillete, J. R. (1974). Bromoibenzene-induced Liver necrosis: Protective role of glutathione and evidence for 3, 4- Bromobenzene oxide as hepatotoxic metabolite. Pharmacology. 1, 151-169.
[23]	Mishra, H. P., Fridovich, I. (1972). The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. The Journal of Biological Chemistry, 247, 3170-3175.
[24]	Luck, H. (1974). Catalase. In: Bergmayer, M. V. (Ed.), Method of Enzymatic Analysis. Verlag Chemic. Academic Press, New York, p. 885.
[25]	Chiu, D. T., Stults, F. H., & Tappel, A. L. (1976). Purification and properties of rat lung soluble glutathione peroxidase. Biochimica et Biophysica Acta (BBA)-Enzymology, 445(3), 558-566.
[26]	Habig, W. H., Pabst, M. J., Jakoby, W. B. (1974). Glutathione S-transferases the first enzymatic step in mercapturic acid formation. Journal of biological Chemistry, 249(22), 7130-7139.
[27]	Tappel, A. L., Zalkin, H. (1959). Inhibition of lipide peroxidation in mitochondria by vitamin E. Archives of Biochemistry and Biophysics, 80(2), 333-336.
[28]	Bancroft, J. D., & Cook, H. C. (1994). Manual of histological techniques and their diagnostic application. Churchill Livingstone, Edinburgh, London, New York, Tokyo 23-26.
[29]	Ratnasooriya, W. D., Ratnayak, S. K., Jayatunga, Y. A. (2002). Effects of pyrethroid insecticide ICON (lambda cyhalothrin) on reproductive competence of male rats. Asian journal of andrology, 4(1), pp. 35-42.
[30]	Darbar, S., Bhattacharya, A., & Chattopadhyay, S. (2010). Ameliorative effect of Livina: apolyherbal preparation on Diclofenac-induced liver injury: a comparison with Silymarin, J. Pharm. Res. 3, 2794–2798.
[31]	Adeyemi, W. J., & Olayaki, L. A. (2018). Diclofenac – induced hepatotoxicity: Low dose of omega-3 fatty acids have more protective effects. Toxicology Reports 5, 90–95.
[32]	Alabi, Q. K., Akomolafe, R. O., Olukiran, O. S., Adeyemi, W. J., Nafiu, A. O., Adefisayo, M. A., Omole, J. G., Kajewole, D. I., & Odujoko, O. O. (2017). The Garcinia kola biflavonoid kolaviron attenuates experimental hepatotoxicity induced by diclofenac. Pathophysiology. 1-10.
[33]	Tsai-Turton, M., Luong, B. T., Tan, Y., & Luderer, U. (2007). Cyclophosphamide-Induced Apoptosis in COV434 Human Granulosa Cells Involves Oxidative Stress and Glutathione Depletion. Toxicol. Sci., 98, 216-230.

Cite This Article

Plain Text BibTeX RIS

APA Style

Mohamed Abd El-Ghany El-Sayed, Omayma El-Sayed Shaltot, Mokhtar Ibrahim Yousef, Entisar Abd El-Mohsen El-Difrawy. (2019). Protective Effect of Aerial Parts of Portulaca oleracea and Ficus carica Leaves Against Diclofenac-Sodium Induced Hepatotoxicity in Rats. Journal of Food and Nutrition Sciences, 7(1), 1-7. https://doi.org/10.11648/j.jfns.20190701.11

Copy | Download

ACS Style

Mohamed Abd El-Ghany El-Sayed; Omayma El-Sayed Shaltot; Mokhtar Ibrahim Yousef; Entisar Abd El-Mohsen El-Difrawy. Protective Effect of Aerial Parts of Portulaca oleracea and Ficus carica Leaves Against Diclofenac-Sodium Induced Hepatotoxicity in Rats. J. Food Nutr. Sci. 2019, 7(1), 1-7. doi: 10.11648/j.jfns.20190701.11

Copy | Download

AMA Style

Mohamed Abd El-Ghany El-Sayed, Omayma El-Sayed Shaltot, Mokhtar Ibrahim Yousef, Entisar Abd El-Mohsen El-Difrawy. Protective Effect of Aerial Parts of Portulaca oleracea and Ficus carica Leaves Against Diclofenac-Sodium Induced Hepatotoxicity in Rats. J Food Nutr Sci. 2019;7(1):1-7. doi: 10.11648/j.jfns.20190701.11

Copy | Download

@article{10.11648/j.jfns.20190701.11,
  author = {Mohamed Abd El-Ghany El-Sayed and Omayma El-Sayed Shaltot and Mokhtar Ibrahim Yousef and Entisar Abd El-Mohsen El-Difrawy},
  title = {Protective Effect of Aerial Parts of Portulaca oleracea and Ficus carica Leaves Against Diclofenac-Sodium Induced Hepatotoxicity in Rats},
  journal = {Journal of Food and Nutrition Sciences},
  volume = {7},
  number = {1},
  pages = {1-7},
  doi = {10.11648/j.jfns.20190701.11},
  url = {https://doi.org/10.11648/j.jfns.20190701.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jfns.20190701.11},
  abstract = {The present experiment is aimed to evaluate the hepatoprotective effects of purslane (PuE) and fig leaves (FlE) extracts on diclofenac-sodium (DS) induced hepatotoxicity in rats. Adult male rats were pretreated orally with PuE and FlE extracts at a dose of 10ml/kg and 200mg/kg body weight, respectively for 14 days. Co-treatment of DS 16mg/kg body weight was given orally for 7days. The present results demonstrated that the treatment with PuE and FlE combination with DS induced a marked improvement in the studied parameters. Data indicated that there were no significant differences in relative liver weight (RLW) and relative kidney weight (RKW) in group treated with PuE and FlE and /or their combination as compared to control. Plasma liver enzyme activities as well as bilirubin levels were increased in the groups receiving diclofenac only or in combination as compared with control group. However, the administration of PuE and FlE ameliorated DS induced hepatotoxicity by improving antioxidant status, decreasing inflammation, lowering TBARS and weakening the adverse effect of diclofenac on hepatic tissues. Liver injury was confirmed by the histological changes. Taken together, the present study concluded that enhancement of antioxidants and promising activity against diclofenac-induced hepatotoxicity may a result for the effect of PuE and FlE.},
 year = {2019}
}

Copy | Download

TY  - JOUR
T1  - Protective Effect of Aerial Parts of Portulaca oleracea and Ficus carica Leaves Against Diclofenac-Sodium Induced Hepatotoxicity in Rats
AU  - Mohamed Abd El-Ghany El-Sayed
AU  - Omayma El-Sayed Shaltot
AU  - Mokhtar Ibrahim Yousef
AU  - Entisar Abd El-Mohsen El-Difrawy
Y1  - 2019/05/23
PY  - 2019
N1  - https://doi.org/10.11648/j.jfns.20190701.11
DO  - 10.11648/j.jfns.20190701.11
T2  - Journal of Food and Nutrition Sciences
JF  - Journal of Food and Nutrition Sciences
JO  - Journal of Food and Nutrition Sciences
SP  - 1
EP  - 7
PB  - Science Publishing Group
SN  - 2330-7293
UR  - https://doi.org/10.11648/j.jfns.20190701.11
AB  - The present experiment is aimed to evaluate the hepatoprotective effects of purslane (PuE) and fig leaves (FlE) extracts on diclofenac-sodium (DS) induced hepatotoxicity in rats. Adult male rats were pretreated orally with PuE and FlE extracts at a dose of 10ml/kg and 200mg/kg body weight, respectively for 14 days. Co-treatment of DS 16mg/kg body weight was given orally for 7days. The present results demonstrated that the treatment with PuE and FlE combination with DS induced a marked improvement in the studied parameters. Data indicated that there were no significant differences in relative liver weight (RLW) and relative kidney weight (RKW) in group treated with PuE and FlE and /or their combination as compared to control. Plasma liver enzyme activities as well as bilirubin levels were increased in the groups receiving diclofenac only or in combination as compared with control group. However, the administration of PuE and FlE ameliorated DS induced hepatotoxicity by improving antioxidant status, decreasing inflammation, lowering TBARS and weakening the adverse effect of diclofenac on hepatic tissues. Liver injury was confirmed by the histological changes. Taken together, the present study concluded that enhancement of antioxidants and promising activity against diclofenac-induced hepatotoxicity may a result for the effect of PuE and FlE.
VL  - 7
IS  - 1
ER  -

Copy | Download

Author Information

Mohamed Abd El-Ghany El-Sayed

Food Technology Department, Institute of Agriculture Research Center, Giza, Egypt
Omayma El-Sayed Shaltot

Food Science Department, Faculty of Agriculture Saba Basha, Alexandria University, Alexandria, Egypt
Mokhtar Ibrahim Yousef

Department of Environmental Studies, Institute of Graduate Studies & Research, Alexandria University, Alexandria, Egypt
Entisar Abd El-Mohsen El-Difrawy

Food Science Department, Faculty of Agriculture Saba Basha, Alexandria University, Alexandria, Egypt

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Mohamed Abd El-Ghany El-Sayed, Omayma El-Sayed Shaltot, Mokhtar Ibrahim Yousef, Entisar Abd El-Mohsen El-Difrawy. (2019). Protective Effect of Aerial Parts of Portulaca oleracea and Ficus carica Leaves Against Diclofenac-Sodium Induced Hepatotoxicity in Rats. Journal of Food and Nutrition Sciences, 7(1), 1-7. https://doi.org/10.11648/j.jfns.20190701.11

Copy | Download

ACS Style

Mohamed Abd El-Ghany El-Sayed; Omayma El-Sayed Shaltot; Mokhtar Ibrahim Yousef; Entisar Abd El-Mohsen El-Difrawy. Protective Effect of Aerial Parts of Portulaca oleracea and Ficus carica Leaves Against Diclofenac-Sodium Induced Hepatotoxicity in Rats. J. Food Nutr. Sci. 2019, 7(1), 1-7. doi: 10.11648/j.jfns.20190701.11

Copy | Download

AMA Style

Mohamed Abd El-Ghany El-Sayed, Omayma El-Sayed Shaltot, Mokhtar Ibrahim Yousef, Entisar Abd El-Mohsen El-Difrawy. Protective Effect of Aerial Parts of Portulaca oleracea and Ficus carica Leaves Against Diclofenac-Sodium Induced Hepatotoxicity in Rats. J Food Nutr Sci. 2019;7(1):1-7. doi: 10.11648/j.jfns.20190701.11

Copy | Download

@article{10.11648/j.jfns.20190701.11,
  author = {Mohamed Abd El-Ghany El-Sayed and Omayma El-Sayed Shaltot and Mokhtar Ibrahim Yousef and Entisar Abd El-Mohsen El-Difrawy},
  title = {Protective Effect of Aerial Parts of Portulaca oleracea and Ficus carica Leaves Against Diclofenac-Sodium Induced Hepatotoxicity in Rats},
  journal = {Journal of Food and Nutrition Sciences},
  volume = {7},
  number = {1},
  pages = {1-7},
  doi = {10.11648/j.jfns.20190701.11},
  url = {https://doi.org/10.11648/j.jfns.20190701.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jfns.20190701.11},
  abstract = {The present experiment is aimed to evaluate the hepatoprotective effects of purslane (PuE) and fig leaves (FlE) extracts on diclofenac-sodium (DS) induced hepatotoxicity in rats. Adult male rats were pretreated orally with PuE and FlE extracts at a dose of 10ml/kg and 200mg/kg body weight, respectively for 14 days. Co-treatment of DS 16mg/kg body weight was given orally for 7days. The present results demonstrated that the treatment with PuE and FlE combination with DS induced a marked improvement in the studied parameters. Data indicated that there were no significant differences in relative liver weight (RLW) and relative kidney weight (RKW) in group treated with PuE and FlE and /or their combination as compared to control. Plasma liver enzyme activities as well as bilirubin levels were increased in the groups receiving diclofenac only or in combination as compared with control group. However, the administration of PuE and FlE ameliorated DS induced hepatotoxicity by improving antioxidant status, decreasing inflammation, lowering TBARS and weakening the adverse effect of diclofenac on hepatic tissues. Liver injury was confirmed by the histological changes. Taken together, the present study concluded that enhancement of antioxidants and promising activity against diclofenac-induced hepatotoxicity may a result for the effect of PuE and FlE.},
 year = {2019}
}

Copy | Download

TY  - JOUR
T1  - Protective Effect of Aerial Parts of Portulaca oleracea and Ficus carica Leaves Against Diclofenac-Sodium Induced Hepatotoxicity in Rats
AU  - Mohamed Abd El-Ghany El-Sayed
AU  - Omayma El-Sayed Shaltot
AU  - Mokhtar Ibrahim Yousef
AU  - Entisar Abd El-Mohsen El-Difrawy
Y1  - 2019/05/23
PY  - 2019
N1  - https://doi.org/10.11648/j.jfns.20190701.11
DO  - 10.11648/j.jfns.20190701.11
T2  - Journal of Food and Nutrition Sciences
JF  - Journal of Food and Nutrition Sciences
JO  - Journal of Food and Nutrition Sciences
SP  - 1
EP  - 7
PB  - Science Publishing Group
SN  - 2330-7293
UR  - https://doi.org/10.11648/j.jfns.20190701.11
AB  - The present experiment is aimed to evaluate the hepatoprotective effects of purslane (PuE) and fig leaves (FlE) extracts on diclofenac-sodium (DS) induced hepatotoxicity in rats. Adult male rats were pretreated orally with PuE and FlE extracts at a dose of 10ml/kg and 200mg/kg body weight, respectively for 14 days. Co-treatment of DS 16mg/kg body weight was given orally for 7days. The present results demonstrated that the treatment with PuE and FlE combination with DS induced a marked improvement in the studied parameters. Data indicated that there were no significant differences in relative liver weight (RLW) and relative kidney weight (RKW) in group treated with PuE and FlE and /or their combination as compared to control. Plasma liver enzyme activities as well as bilirubin levels were increased in the groups receiving diclofenac only or in combination as compared with control group. However, the administration of PuE and FlE ameliorated DS induced hepatotoxicity by improving antioxidant status, decreasing inflammation, lowering TBARS and weakening the adverse effect of diclofenac on hepatic tissues. Liver injury was confirmed by the histological changes. Taken together, the present study concluded that enhancement of antioxidants and promising activity against diclofenac-induced hepatotoxicity may a result for the effect of PuE and FlE.
VL  - 7
IS  - 1
ER  -

Copy | Download