Fabrication of Electrospun Polycaprolactone/Gelatin Composite Nanofibrous Scaffolds with Cellular Responses

Jaianand Kannaiyan; Saurabh Khare; Suriya Narayanan; Firdosh Mahuvawalla

doi:doi:10.11648/j.nano.20190702.11

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Fabrication of Electrospun Polycaprolactone/Gelatin Composite Nanofibrous Scaffolds with Cellular Responses

Jaianand Kannaiyan, Saurabh Khare, Suriya Narayanan, Firdosh Mahuvawalla

Published in American Journal of Nano Research and Applications (Volume 7, Issue 2)

Received: 9 October 2019 Accepted: 4 November 2019 Published: 8 November 2019

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Abstract

Numerous wound care products have been investigated for skin tissue engineering. Factors which influence to skin substitute selection are condition of patient, wound depth, infection in wound. Other factors which also play role in deciding skin substitute are cost, its availability, ease of storage, required operative interventions. With perspective of Indian market, commercial available skin substitutes are either costly or their availability restricted to major cities only. A cost effective skin substitute is strongly needed to heal wounds with minimal scarring and maximum function. The aim of this study was to investigate the possibility of synthetic scaffold loaded with Wharton’s jelly derived Mesenchymal stem cells and to access the role of scaffolds in proliferation and differentiation of MSCs in-vitro, in order to achieve for the healing of wound graft substitutes with improved biological properties. As a result, WJ-MSCs were isolated, harvested and seeded on the surface of the fabricated PCL/GE nanofibrous scaffold. The biological properties and growth of MSCs were studied for anti-inflammation, cytotoxicity, cell proliferation, and SEM analysis indicated that the fabricated synthetic scaffold supported cells attachment, viability, and proliferation of cells. The characterization studies of nanofibers were studied for ATR-FTIR, XRD, TEM, viscosity, and degradation studies suggest that the nanofibrous scaffold loaded with stem cells could be an excellent tissue-engineered skin base for wound healing and skin regeneration.

Published in	American Journal of Nano Research and Applications (Volume 7, Issue 2)
DOI	10.11648/j.nano.20190702.11
Page(s)	11-20
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Electrospinning, Polycaprolactone (PCL), Gelatin (GE), Nanofibrous Scaffold, Mesenchymal Stem Cells (MSCs)

References

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APA Style

Jaianand Kannaiyan, Saurabh Khare, Suriya Narayanan, Firdosh Mahuvawalla. (2019). Fabrication of Electrospun Polycaprolactone/Gelatin Composite Nanofibrous Scaffolds with Cellular Responses. American Journal of Nano Research and Applications, 7(2), 11-20. https://doi.org/10.11648/j.nano.20190702.11

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ACS Style

Jaianand Kannaiyan; Saurabh Khare; Suriya Narayanan; Firdosh Mahuvawalla. Fabrication of Electrospun Polycaprolactone/Gelatin Composite Nanofibrous Scaffolds with Cellular Responses. Am. J. Nano Res. Appl. 2019, 7(2), 11-20. doi: 10.11648/j.nano.20190702.11

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AMA Style

Jaianand Kannaiyan, Saurabh Khare, Suriya Narayanan, Firdosh Mahuvawalla. Fabrication of Electrospun Polycaprolactone/Gelatin Composite Nanofibrous Scaffolds with Cellular Responses. Am J Nano Res Appl. 2019;7(2):11-20. doi: 10.11648/j.nano.20190702.11

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@article{10.11648/j.nano.20190702.11,
  author = {Jaianand Kannaiyan and Saurabh Khare and Suriya Narayanan and Firdosh Mahuvawalla},
  title = {Fabrication of Electrospun Polycaprolactone/Gelatin Composite Nanofibrous Scaffolds with Cellular Responses},
  journal = {American Journal of Nano Research and Applications},
  volume = {7},
  number = {2},
  pages = {11-20},
  doi = {10.11648/j.nano.20190702.11},
  url = {https://doi.org/10.11648/j.nano.20190702.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.nano.20190702.11},
  abstract = {Numerous wound care products have been investigated for skin tissue engineering. Factors which influence to skin substitute selection are condition of patient, wound depth, infection in wound. Other factors which also play role in deciding skin substitute are cost, its availability, ease of storage, required operative interventions. With perspective of Indian market, commercial available skin substitutes are either costly or their availability restricted to major cities only. A cost effective skin substitute is strongly needed to heal wounds with minimal scarring and maximum function. The aim of this study was to investigate the possibility of synthetic scaffold loaded with Wharton’s jelly derived Mesenchymal stem cells and to access the role of scaffolds in proliferation and differentiation of MSCs in-vitro, in order to achieve for the healing of wound graft substitutes with improved biological properties. As a result, WJ-MSCs were isolated, harvested and seeded on the surface of the fabricated PCL/GE nanofibrous scaffold. The biological properties and growth of MSCs were studied for anti-inflammation, cytotoxicity, cell proliferation, and SEM analysis indicated that the fabricated synthetic scaffold supported cells attachment, viability, and proliferation of cells. The characterization studies of nanofibers were studied for ATR-FTIR, XRD, TEM, viscosity, and degradation studies suggest that the nanofibrous scaffold loaded with stem cells could be an excellent tissue-engineered skin base for wound healing and skin regeneration.},
 year = {2019}
}

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TY  - JOUR
T1  - Fabrication of Electrospun Polycaprolactone/Gelatin Composite Nanofibrous Scaffolds with Cellular Responses
AU  - Jaianand Kannaiyan
AU  - Saurabh Khare
AU  - Suriya Narayanan
AU  - Firdosh Mahuvawalla
Y1  - 2019/11/08
PY  - 2019
N1  - https://doi.org/10.11648/j.nano.20190702.11
DO  - 10.11648/j.nano.20190702.11
T2  - American Journal of Nano Research and Applications
JF  - American Journal of Nano Research and Applications
JO  - American Journal of Nano Research and Applications
SP  - 11
EP  - 20
PB  - Science Publishing Group
SN  - 2575-3738
UR  - https://doi.org/10.11648/j.nano.20190702.11
AB  - Numerous wound care products have been investigated for skin tissue engineering. Factors which influence to skin substitute selection are condition of patient, wound depth, infection in wound. Other factors which also play role in deciding skin substitute are cost, its availability, ease of storage, required operative interventions. With perspective of Indian market, commercial available skin substitutes are either costly or their availability restricted to major cities only. A cost effective skin substitute is strongly needed to heal wounds with minimal scarring and maximum function. The aim of this study was to investigate the possibility of synthetic scaffold loaded with Wharton’s jelly derived Mesenchymal stem cells and to access the role of scaffolds in proliferation and differentiation of MSCs in-vitro, in order to achieve for the healing of wound graft substitutes with improved biological properties. As a result, WJ-MSCs were isolated, harvested and seeded on the surface of the fabricated PCL/GE nanofibrous scaffold. The biological properties and growth of MSCs were studied for anti-inflammation, cytotoxicity, cell proliferation, and SEM analysis indicated that the fabricated synthetic scaffold supported cells attachment, viability, and proliferation of cells. The characterization studies of nanofibers were studied for ATR-FTIR, XRD, TEM, viscosity, and degradation studies suggest that the nanofibrous scaffold loaded with stem cells could be an excellent tissue-engineered skin base for wound healing and skin regeneration.
VL  - 7
IS  - 2
ER  -

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Author Information

Jaianand Kannaiyan

CelluGen Biotech Private Limited, Research and Development, Gurgaon, Haryana, India
Saurabh Khare

CelluGen Biotech Private Limited, Research and Development, Gurgaon, Haryana, India
Suriya Narayanan

CelluGen Biotech Private Limited, Research and Development, Gurgaon, Haryana, India
Firdosh Mahuvawalla

CelluGen Biotech Private Limited, Research and Development, Gurgaon, Haryana, India

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APA Style

Jaianand Kannaiyan, Saurabh Khare, Suriya Narayanan, Firdosh Mahuvawalla. (2019). Fabrication of Electrospun Polycaprolactone/Gelatin Composite Nanofibrous Scaffolds with Cellular Responses. American Journal of Nano Research and Applications, 7(2), 11-20. https://doi.org/10.11648/j.nano.20190702.11

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ACS Style

Jaianand Kannaiyan; Saurabh Khare; Suriya Narayanan; Firdosh Mahuvawalla. Fabrication of Electrospun Polycaprolactone/Gelatin Composite Nanofibrous Scaffolds with Cellular Responses. Am. J. Nano Res. Appl. 2019, 7(2), 11-20. doi: 10.11648/j.nano.20190702.11

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AMA Style

Jaianand Kannaiyan, Saurabh Khare, Suriya Narayanan, Firdosh Mahuvawalla. Fabrication of Electrospun Polycaprolactone/Gelatin Composite Nanofibrous Scaffolds with Cellular Responses. Am J Nano Res Appl. 2019;7(2):11-20. doi: 10.11648/j.nano.20190702.11

Copy | Download

@article{10.11648/j.nano.20190702.11,
  author = {Jaianand Kannaiyan and Saurabh Khare and Suriya Narayanan and Firdosh Mahuvawalla},
  title = {Fabrication of Electrospun Polycaprolactone/Gelatin Composite Nanofibrous Scaffolds with Cellular Responses},
  journal = {American Journal of Nano Research and Applications},
  volume = {7},
  number = {2},
  pages = {11-20},
  doi = {10.11648/j.nano.20190702.11},
  url = {https://doi.org/10.11648/j.nano.20190702.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.nano.20190702.11},
  abstract = {Numerous wound care products have been investigated for skin tissue engineering. Factors which influence to skin substitute selection are condition of patient, wound depth, infection in wound. Other factors which also play role in deciding skin substitute are cost, its availability, ease of storage, required operative interventions. With perspective of Indian market, commercial available skin substitutes are either costly or their availability restricted to major cities only. A cost effective skin substitute is strongly needed to heal wounds with minimal scarring and maximum function. The aim of this study was to investigate the possibility of synthetic scaffold loaded with Wharton’s jelly derived Mesenchymal stem cells and to access the role of scaffolds in proliferation and differentiation of MSCs in-vitro, in order to achieve for the healing of wound graft substitutes with improved biological properties. As a result, WJ-MSCs were isolated, harvested and seeded on the surface of the fabricated PCL/GE nanofibrous scaffold. The biological properties and growth of MSCs were studied for anti-inflammation, cytotoxicity, cell proliferation, and SEM analysis indicated that the fabricated synthetic scaffold supported cells attachment, viability, and proliferation of cells. The characterization studies of nanofibers were studied for ATR-FTIR, XRD, TEM, viscosity, and degradation studies suggest that the nanofibrous scaffold loaded with stem cells could be an excellent tissue-engineered skin base for wound healing and skin regeneration.},
 year = {2019}
}

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TY  - JOUR
T1  - Fabrication of Electrospun Polycaprolactone/Gelatin Composite Nanofibrous Scaffolds with Cellular Responses
AU  - Jaianand Kannaiyan
AU  - Saurabh Khare
AU  - Suriya Narayanan
AU  - Firdosh Mahuvawalla
Y1  - 2019/11/08
PY  - 2019
N1  - https://doi.org/10.11648/j.nano.20190702.11
DO  - 10.11648/j.nano.20190702.11
T2  - American Journal of Nano Research and Applications
JF  - American Journal of Nano Research and Applications
JO  - American Journal of Nano Research and Applications
SP  - 11
EP  - 20
PB  - Science Publishing Group
SN  - 2575-3738
UR  - https://doi.org/10.11648/j.nano.20190702.11
AB  - Numerous wound care products have been investigated for skin tissue engineering. Factors which influence to skin substitute selection are condition of patient, wound depth, infection in wound. Other factors which also play role in deciding skin substitute are cost, its availability, ease of storage, required operative interventions. With perspective of Indian market, commercial available skin substitutes are either costly or their availability restricted to major cities only. A cost effective skin substitute is strongly needed to heal wounds with minimal scarring and maximum function. The aim of this study was to investigate the possibility of synthetic scaffold loaded with Wharton’s jelly derived Mesenchymal stem cells and to access the role of scaffolds in proliferation and differentiation of MSCs in-vitro, in order to achieve for the healing of wound graft substitutes with improved biological properties. As a result, WJ-MSCs were isolated, harvested and seeded on the surface of the fabricated PCL/GE nanofibrous scaffold. The biological properties and growth of MSCs were studied for anti-inflammation, cytotoxicity, cell proliferation, and SEM analysis indicated that the fabricated synthetic scaffold supported cells attachment, viability, and proliferation of cells. The characterization studies of nanofibers were studied for ATR-FTIR, XRD, TEM, viscosity, and degradation studies suggest that the nanofibrous scaffold loaded with stem cells could be an excellent tissue-engineered skin base for wound healing and skin regeneration.
VL  - 7
IS  - 2
ER  -

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