The <i>mtlD</i> Gene-overexpressed Transgenic Wheat Tolerates Salt Stress Through Accumulation of Mannitol and Sugars

Mohamed Ahmed Seif El-Yazal; Hala Fawzi Eissa; Safia Mahmoud Abd El-Mageed Ahmed; Saad Mohamed Howladar; Safi-naz Sabet Zaki; Mostafa Mohamed Rady

doi:doi:10.11648/j.plant.20160406.15

The mtlD Gene-overexpressed Transgenic Wheat Tolerates Salt Stress Through Accumulation of Mannitol and Sugars

Mohamed Ahmed Seif El-Yazal, Hala Fawzi Eissa, Safia Mahmoud Abd El-Mageed Ahmed, Saad Mohamed Howladar, Safi-naz Sabet Zaki, Mostafa Mohamed Rady

Published in Plant (Volume 4, Issue 6)

Received: 17 September 2016 Accepted: 12 October 2016 Published: 31 October 2016

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Abstract

The mtlD gene-contained transgenic wheat has established the role of mannitol and sugars accumulation in alleviating the abiotic stresses, including salinity. This study was conducted to determine whether the 85 mM NaCl-salinity could be tolerated by wheat (genotype 235/3) plants of which seeds were transformed with mtlD gene (from Escherichia coli). The effects of mtlD gene transformation into wheat seeds on growth traits, physio-biochemical attributes, and yield and its quality of transgenic wheat genotype were investigated compared to non-transgenic wheat genotype under 85 mM NaCl-salinity. Results showed that mtlD gene-contained transgenic plants had improved salt tolerance over non-transgenics, showing by better growth traits (i.e., number of leaves and leaf area per plant, root system size and plant dry weights), physio-biochemical attributes (i.e., levels of leaf chlorophylls, shoot free proline, total soluble sugars, soluble sugar fractions and mannitol, activities of enzymatic and non-enzymatic antioxidants, and contents of nutrient elements), yield (i.e., number of spikes and grain weight per plant, and 1000-grain weight) and yield quality (i.e., grain contents of starch, protein and soluble sugars). The mtlD gene transformation into wheat seeds appears to a better strategy to increase salt tolerance of plants through increased performance of mannitol and sugar accumulation, showing more of their salt stress-protecting role. The best performing mtlD transgenics could be incorporated in a breeding program to accumulate transgenes for stress tolerance in elite wheat genotypes in a step to commercialize these transgenics with the proper level of salt tolerance.

Published in	Plant (Volume 4, Issue 6)
DOI	10.11648/j.plant.20160406.15
Page(s)	78-90
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Physio-Biochemical Attributes, Salt Stress, Transgenic Wheat, Yield and Its Quality

References

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Mohamed Ahmed Seif El-Yazal, Hala Fawzi Eissa, Safia Mahmoud Abd El-Mageed Ahmed, Saad Mohamed Howladar, Safi-naz Sabet Zaki, et al. (2016). The mtlD Gene-overexpressed Transgenic Wheat Tolerates Salt Stress Through Accumulation of Mannitol and Sugars. Plant, 4(6), 78-90. https://doi.org/10.11648/j.plant.20160406.15

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Mohamed Ahmed Seif El-Yazal; Hala Fawzi Eissa; Safia Mahmoud Abd El-Mageed Ahmed; Saad Mohamed Howladar; Safi-naz Sabet Zaki, et al. The mtlD Gene-overexpressed Transgenic Wheat Tolerates Salt Stress Through Accumulation of Mannitol and Sugars. Plant. 2016, 4(6), 78-90. doi: 10.11648/j.plant.20160406.15

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Mohamed Ahmed Seif El-Yazal, Hala Fawzi Eissa, Safia Mahmoud Abd El-Mageed Ahmed, Saad Mohamed Howladar, Safi-naz Sabet Zaki, et al. The mtlD Gene-overexpressed Transgenic Wheat Tolerates Salt Stress Through Accumulation of Mannitol and Sugars. Plant. 2016;4(6):78-90. doi: 10.11648/j.plant.20160406.15

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@article{10.11648/j.plant.20160406.15,
  author = {Mohamed Ahmed Seif El-Yazal and Hala Fawzi Eissa and Safia Mahmoud Abd El-Mageed Ahmed and Saad Mohamed Howladar and Safi-naz Sabet Zaki and Mostafa Mohamed Rady},
  title = {The mtlD Gene-overexpressed Transgenic Wheat Tolerates Salt Stress Through Accumulation of Mannitol and Sugars},
  journal = {Plant},
  volume = {4},
  number = {6},
  pages = {78-90},
  doi = {10.11648/j.plant.20160406.15},
  url = {https://doi.org/10.11648/j.plant.20160406.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.plant.20160406.15},
  abstract = {The mtlD gene-contained transgenic wheat has established the role of mannitol and sugars accumulation in alleviating the abiotic stresses, including salinity. This study was conducted to determine whether the 85 mM NaCl-salinity could be tolerated by wheat (genotype 235/3) plants of which seeds were transformed with mtlD gene (from Escherichia coli). The effects of mtlD gene transformation into wheat seeds on growth traits, physio-biochemical attributes, and yield and its quality of transgenic wheat genotype were investigated compared to non-transgenic wheat genotype under 85 mM NaCl-salinity. Results showed that mtlD gene-contained transgenic plants had improved salt tolerance over non-transgenics, showing by better growth traits (i.e., number of leaves and leaf area per plant, root system size and plant dry weights), physio-biochemical attributes (i.e., levels of leaf chlorophylls, shoot free proline, total soluble sugars, soluble sugar fractions and mannitol, activities of enzymatic and non-enzymatic antioxidants, and contents of nutrient elements), yield (i.e., number of spikes and grain weight per plant, and 1000-grain weight) and yield quality (i.e., grain contents of starch, protein and soluble sugars). The mtlD gene transformation into wheat seeds appears to a better strategy to increase salt tolerance of plants through increased performance of mannitol and sugar accumulation, showing more of their salt stress-protecting role. The best performing mtlD transgenics could be incorporated in a breeding program to accumulate transgenes for stress tolerance in elite wheat genotypes in a step to commercialize these transgenics with the proper level of salt tolerance.},
 year = {2016}
}

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TY  - JOUR
T1  - The mtlD Gene-overexpressed Transgenic Wheat Tolerates Salt Stress Through Accumulation of Mannitol and Sugars
AU  - Mohamed Ahmed Seif El-Yazal
AU  - Hala Fawzi Eissa
AU  - Safia Mahmoud Abd El-Mageed Ahmed
AU  - Saad Mohamed Howladar
AU  - Safi-naz Sabet Zaki
AU  - Mostafa Mohamed Rady
Y1  - 2016/10/31
PY  - 2016
N1  - https://doi.org/10.11648/j.plant.20160406.15
DO  - 10.11648/j.plant.20160406.15
T2  - Plant
JF  - Plant
JO  - Plant
SP  - 78
EP  - 90
PB  - Science Publishing Group
SN  - 2331-0677
UR  - https://doi.org/10.11648/j.plant.20160406.15
AB  - The mtlD gene-contained transgenic wheat has established the role of mannitol and sugars accumulation in alleviating the abiotic stresses, including salinity. This study was conducted to determine whether the 85 mM NaCl-salinity could be tolerated by wheat (genotype 235/3) plants of which seeds were transformed with mtlD gene (from Escherichia coli). The effects of mtlD gene transformation into wheat seeds on growth traits, physio-biochemical attributes, and yield and its quality of transgenic wheat genotype were investigated compared to non-transgenic wheat genotype under 85 mM NaCl-salinity. Results showed that mtlD gene-contained transgenic plants had improved salt tolerance over non-transgenics, showing by better growth traits (i.e., number of leaves and leaf area per plant, root system size and plant dry weights), physio-biochemical attributes (i.e., levels of leaf chlorophylls, shoot free proline, total soluble sugars, soluble sugar fractions and mannitol, activities of enzymatic and non-enzymatic antioxidants, and contents of nutrient elements), yield (i.e., number of spikes and grain weight per plant, and 1000-grain weight) and yield quality (i.e., grain contents of starch, protein and soluble sugars). The mtlD gene transformation into wheat seeds appears to a better strategy to increase salt tolerance of plants through increased performance of mannitol and sugar accumulation, showing more of their salt stress-protecting role. The best performing mtlD transgenics could be incorporated in a breeding program to accumulate transgenes for stress tolerance in elite wheat genotypes in a step to commercialize these transgenics with the proper level of salt tolerance.
VL  - 4
IS  - 6
ER  -

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Author Information

Mohamed Ahmed Seif El-Yazal

Botany Department, Faculty of Agriculture, Fayoum University, Fayoum, Egypt
Hala Fawzi Eissa

Agricultural Genetic Engineering Research Institute (AGERI), Agriculture Research Center (ARC), Giza, Egypt
Safia Mahmoud Abd El-Mageed Ahmed

Botany Department, Faculty of Agriculture, Fayoum University, Fayoum, Egypt
Saad Mohamed Howladar

Biology Department, Faculty of Sciences, Albaha University, Albaha, Saudi Arabia
Safi-naz Sabet Zaki

Department of Water Relations and Field Irrigation, National Research Centre, Dokki, Cairo, Egypt
Mostafa Mohamed Rady

Botany Department, Faculty of Agriculture, Fayoum University, Fayoum, Egypt

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Mohamed Ahmed Seif El-Yazal, Hala Fawzi Eissa, Safia Mahmoud Abd El-Mageed Ahmed, Saad Mohamed Howladar, Safi-naz Sabet Zaki, et al. (2016). The mtlD Gene-overexpressed Transgenic Wheat Tolerates Salt Stress Through Accumulation of Mannitol and Sugars. Plant, 4(6), 78-90. https://doi.org/10.11648/j.plant.20160406.15

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Mohamed Ahmed Seif El-Yazal; Hala Fawzi Eissa; Safia Mahmoud Abd El-Mageed Ahmed; Saad Mohamed Howladar; Safi-naz Sabet Zaki, et al. The mtlD Gene-overexpressed Transgenic Wheat Tolerates Salt Stress Through Accumulation of Mannitol and Sugars. Plant. 2016, 4(6), 78-90. doi: 10.11648/j.plant.20160406.15

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AMA Style

Mohamed Ahmed Seif El-Yazal, Hala Fawzi Eissa, Safia Mahmoud Abd El-Mageed Ahmed, Saad Mohamed Howladar, Safi-naz Sabet Zaki, et al. The mtlD Gene-overexpressed Transgenic Wheat Tolerates Salt Stress Through Accumulation of Mannitol and Sugars. Plant. 2016;4(6):78-90. doi: 10.11648/j.plant.20160406.15

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@article{10.11648/j.plant.20160406.15,
  author = {Mohamed Ahmed Seif El-Yazal and Hala Fawzi Eissa and Safia Mahmoud Abd El-Mageed Ahmed and Saad Mohamed Howladar and Safi-naz Sabet Zaki and Mostafa Mohamed Rady},
  title = {The mtlD Gene-overexpressed Transgenic Wheat Tolerates Salt Stress Through Accumulation of Mannitol and Sugars},
  journal = {Plant},
  volume = {4},
  number = {6},
  pages = {78-90},
  doi = {10.11648/j.plant.20160406.15},
  url = {https://doi.org/10.11648/j.plant.20160406.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.plant.20160406.15},
  abstract = {The mtlD gene-contained transgenic wheat has established the role of mannitol and sugars accumulation in alleviating the abiotic stresses, including salinity. This study was conducted to determine whether the 85 mM NaCl-salinity could be tolerated by wheat (genotype 235/3) plants of which seeds were transformed with mtlD gene (from Escherichia coli). The effects of mtlD gene transformation into wheat seeds on growth traits, physio-biochemical attributes, and yield and its quality of transgenic wheat genotype were investigated compared to non-transgenic wheat genotype under 85 mM NaCl-salinity. Results showed that mtlD gene-contained transgenic plants had improved salt tolerance over non-transgenics, showing by better growth traits (i.e., number of leaves and leaf area per plant, root system size and plant dry weights), physio-biochemical attributes (i.e., levels of leaf chlorophylls, shoot free proline, total soluble sugars, soluble sugar fractions and mannitol, activities of enzymatic and non-enzymatic antioxidants, and contents of nutrient elements), yield (i.e., number of spikes and grain weight per plant, and 1000-grain weight) and yield quality (i.e., grain contents of starch, protein and soluble sugars). The mtlD gene transformation into wheat seeds appears to a better strategy to increase salt tolerance of plants through increased performance of mannitol and sugar accumulation, showing more of their salt stress-protecting role. The best performing mtlD transgenics could be incorporated in a breeding program to accumulate transgenes for stress tolerance in elite wheat genotypes in a step to commercialize these transgenics with the proper level of salt tolerance.},
 year = {2016}
}

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TY  - JOUR
T1  - The mtlD Gene-overexpressed Transgenic Wheat Tolerates Salt Stress Through Accumulation of Mannitol and Sugars
AU  - Mohamed Ahmed Seif El-Yazal
AU  - Hala Fawzi Eissa
AU  - Safia Mahmoud Abd El-Mageed Ahmed
AU  - Saad Mohamed Howladar
AU  - Safi-naz Sabet Zaki
AU  - Mostafa Mohamed Rady
Y1  - 2016/10/31
PY  - 2016
N1  - https://doi.org/10.11648/j.plant.20160406.15
DO  - 10.11648/j.plant.20160406.15
T2  - Plant
JF  - Plant
JO  - Plant
SP  - 78
EP  - 90
PB  - Science Publishing Group
SN  - 2331-0677
UR  - https://doi.org/10.11648/j.plant.20160406.15
AB  - The mtlD gene-contained transgenic wheat has established the role of mannitol and sugars accumulation in alleviating the abiotic stresses, including salinity. This study was conducted to determine whether the 85 mM NaCl-salinity could be tolerated by wheat (genotype 235/3) plants of which seeds were transformed with mtlD gene (from Escherichia coli). The effects of mtlD gene transformation into wheat seeds on growth traits, physio-biochemical attributes, and yield and its quality of transgenic wheat genotype were investigated compared to non-transgenic wheat genotype under 85 mM NaCl-salinity. Results showed that mtlD gene-contained transgenic plants had improved salt tolerance over non-transgenics, showing by better growth traits (i.e., number of leaves and leaf area per plant, root system size and plant dry weights), physio-biochemical attributes (i.e., levels of leaf chlorophylls, shoot free proline, total soluble sugars, soluble sugar fractions and mannitol, activities of enzymatic and non-enzymatic antioxidants, and contents of nutrient elements), yield (i.e., number of spikes and grain weight per plant, and 1000-grain weight) and yield quality (i.e., grain contents of starch, protein and soluble sugars). The mtlD gene transformation into wheat seeds appears to a better strategy to increase salt tolerance of plants through increased performance of mannitol and sugar accumulation, showing more of their salt stress-protecting role. The best performing mtlD transgenics could be incorporated in a breeding program to accumulate transgenes for stress tolerance in elite wheat genotypes in a step to commercialize these transgenics with the proper level of salt tolerance.
VL  - 4
IS  - 6
ER  -

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