Gap Filling for a Human MHC Haplotype Sequence

Yuanwei Zhang; Tao Zhang; Zuhong Lu

doi:doi:10.11648/j.ajls.20160406.12

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Gap Filling for a Human MHC Haplotype Sequence

Yuanwei Zhang, Tao Zhang, Zuhong Lu

Published in American Journal of Life Sciences (Volume 4, Issue 6)

Received: 29 November 2016 Published: 1 December 2016

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Abstract

The major histocompatibility complex (MHC) is recognized as the most variable region in the human genome and has susceptibility to > 100 diseases. We constructed a complete MHC haplotype sequence of MCF cell line by gap filling based on whole genome sequencing (WGS) data. Gaps spanning ~ 1 Mb were filled and 31 genes were annotated in these gaps. This sequence could be used as reference to identify disease associations within this haplotype or similar haplotypes. The method for gap filling can be applied to other MHC haplotypes or other genomic region.

Published in	American Journal of Life Sciences (Volume 4, Issue 6)
DOI	10.11648/j.ajls.20160406.12
Page(s)	146-151
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Gap Filling, MHC, Haplotype, WGS

References

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Yuanwei Zhang, Tao Zhang, Zuhong Lu. (2016). Gap Filling for a Human MHC Haplotype Sequence. American Journal of Life Sciences, 4(6), 146-151. https://doi.org/10.11648/j.ajls.20160406.12

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ACS Style

Yuanwei Zhang; Tao Zhang; Zuhong Lu. Gap Filling for a Human MHC Haplotype Sequence. Am. J. Life Sci. 2016, 4(6), 146-151. doi: 10.11648/j.ajls.20160406.12

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AMA Style

Yuanwei Zhang, Tao Zhang, Zuhong Lu. Gap Filling for a Human MHC Haplotype Sequence. Am J Life Sci. 2016;4(6):146-151. doi: 10.11648/j.ajls.20160406.12

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@article{10.11648/j.ajls.20160406.12,
  author = {Yuanwei Zhang and Tao Zhang and Zuhong Lu},
  title = {Gap Filling for a Human MHC Haplotype Sequence},
  journal = {American Journal of Life Sciences},
  volume = {4},
  number = {6},
  pages = {146-151},
  doi = {10.11648/j.ajls.20160406.12},
  url = {https://doi.org/10.11648/j.ajls.20160406.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20160406.12},
  abstract = {The major histocompatibility complex (MHC) is recognized as the most variable region in the human genome and has susceptibility to > 100 diseases. We constructed a complete MHC haplotype sequence of MCF cell line by gap filling based on whole genome sequencing (WGS) data. Gaps spanning ~ 1 Mb were filled and 31 genes were annotated in these gaps. This sequence could be used as reference to identify disease associations within this haplotype or similar haplotypes. The method for gap filling can be applied to other MHC haplotypes or other genomic region.},
 year = {2016}
}

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TY  - JOUR
T1  - Gap Filling for a Human MHC Haplotype Sequence
AU  - Yuanwei Zhang
AU  - Tao Zhang
AU  - Zuhong Lu
Y1  - 2016/12/01
PY  - 2016
N1  - https://doi.org/10.11648/j.ajls.20160406.12
DO  - 10.11648/j.ajls.20160406.12
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 146
EP  - 151
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.20160406.12
AB  - The major histocompatibility complex (MHC) is recognized as the most variable region in the human genome and has susceptibility to > 100 diseases. We constructed a complete MHC haplotype sequence of MCF cell line by gap filling based on whole genome sequencing (WGS) data. Gaps spanning ~ 1 Mb were filled and 31 genes were annotated in these gaps. This sequence could be used as reference to identify disease associations within this haplotype or similar haplotypes. The method for gap filling can be applied to other MHC haplotypes or other genomic region.
VL  - 4
IS  - 6
ER  -

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Author Information

Yuanwei Zhang

School of Biological Science and Medical Engineering, Southeast University, Nanjing, China
Tao Zhang

BGI-Shenzhen, Shenzhen, China
Zuhong Lu

School of Biological Science and Medical Engineering, Southeast University, Nanjing, China

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APA Style

Yuanwei Zhang, Tao Zhang, Zuhong Lu. (2016). Gap Filling for a Human MHC Haplotype Sequence. American Journal of Life Sciences, 4(6), 146-151. https://doi.org/10.11648/j.ajls.20160406.12

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ACS Style

Yuanwei Zhang; Tao Zhang; Zuhong Lu. Gap Filling for a Human MHC Haplotype Sequence. Am. J. Life Sci. 2016, 4(6), 146-151. doi: 10.11648/j.ajls.20160406.12

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AMA Style

Yuanwei Zhang, Tao Zhang, Zuhong Lu. Gap Filling for a Human MHC Haplotype Sequence. Am J Life Sci. 2016;4(6):146-151. doi: 10.11648/j.ajls.20160406.12

Copy | Download

@article{10.11648/j.ajls.20160406.12,
  author = {Yuanwei Zhang and Tao Zhang and Zuhong Lu},
  title = {Gap Filling for a Human MHC Haplotype Sequence},
  journal = {American Journal of Life Sciences},
  volume = {4},
  number = {6},
  pages = {146-151},
  doi = {10.11648/j.ajls.20160406.12},
  url = {https://doi.org/10.11648/j.ajls.20160406.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20160406.12},
  abstract = {The major histocompatibility complex (MHC) is recognized as the most variable region in the human genome and has susceptibility to > 100 diseases. We constructed a complete MHC haplotype sequence of MCF cell line by gap filling based on whole genome sequencing (WGS) data. Gaps spanning ~ 1 Mb were filled and 31 genes were annotated in these gaps. This sequence could be used as reference to identify disease associations within this haplotype or similar haplotypes. The method for gap filling can be applied to other MHC haplotypes or other genomic region.},
 year = {2016}
}

Copy | Download

TY  - JOUR
T1  - Gap Filling for a Human MHC Haplotype Sequence
AU  - Yuanwei Zhang
AU  - Tao Zhang
AU  - Zuhong Lu
Y1  - 2016/12/01
PY  - 2016
N1  - https://doi.org/10.11648/j.ajls.20160406.12
DO  - 10.11648/j.ajls.20160406.12
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 146
EP  - 151
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.20160406.12
AB  - The major histocompatibility complex (MHC) is recognized as the most variable region in the human genome and has susceptibility to > 100 diseases. We constructed a complete MHC haplotype sequence of MCF cell line by gap filling based on whole genome sequencing (WGS) data. Gaps spanning ~ 1 Mb were filled and 31 genes were annotated in these gaps. This sequence could be used as reference to identify disease associations within this haplotype or similar haplotypes. The method for gap filling can be applied to other MHC haplotypes or other genomic region.
VL  - 4
IS  - 6
ER  -

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